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PMID: 1856169 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Rifampin-resistant replication of pBR322 derivatives in Escherichia coli cells induced for the SOS response.

Journal of bacteriology ·Vol. 173 ·No. 15 ·1991-08-00 ·Pages 4736-41

Magee TR, Kogoma T

Abstract

Replication of plasmid pBR322 in Escherichia coli cells normally requires RNA synthesis and thus is sensitive to rifampin, an inhibitor of RNA polymerase. In cells induced for the SOS response, however, derivatives of pBR322 were found to replicate in the presence of rifampin. This rifampin-resistant replication of pBR322 requires the insertion of certain sequences of DNA. The replication depends on recF+ and DNA polymerase I.

Related Genes
recF
MeSH Terms
Chromosomes, Bacterial DNA Polymerase I/metabolism DNA Replication DNA, Bacterial/biosynthesis Drug Resistance, Microbial/genetics Escherichia coli/drug effects,enzymology,genetics Plasmids/drug effects Replicon Rifampin/pharmacology SOS Response, Genetics/drug effects Sequence Homology, Nucleic Acid
Chemicals
DNA, Bacterial DNA Polymerase I Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Magee T R
Department of Biology, University of New Mexico, Albuquerque 87131.
Kogoma T
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.15.4736-4741.1991
Published
1991-08-00
Pages
4736-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208151
Subset
IM
Grants
FIC NIH HHS · F06 TWO1443 · United States
NIGMS NIH HHS · GM22092 · United States
External Links
PubMed source DOI: 10.1128/jb.173.15.4736-4741.1991 PMC Full Text View journal details
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