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PMID: 6395859 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS.

The Biochemical journal ·Vol. 224 ·No. 3 ·1984-12-15 ·Pages 799-815

Walker JE, Gay NJ, Saraste M, Eberle AN

Abstract

The nucleotide sequence is described of a region of the Escherichia coli chromosome extending from oriC to phoS that also includes the loci gid, unc and glmS. Taken with known sequences for asnA and phoS this completes the sequence of a segment of about 17 kilobases or 0.4 min of the E. coli genome. Sequences that are probably transcriptional promoters for unc and phoS can be detected and the identity of the unc promoter has been confirmed by experiments in vitro with RNA polymerase. Upstream of the promoter sequence is an extensive region that appears to be non-coding. Conserved sequences are found that may serve to concentrate RNA polymerase in the vicinity of the unc promoter. Hairpin loop structures resembling known rho-independent transcription termination signals are evident following the unc operon and glmS. The glmS gene encoding the amidotransferase, glucosamine synthetase, has been identified by homology with glutamine 5-phosphoribosylpyrophosphate amidotransferase.

MeSH Terms
Amidophosphoribosyltransferase/genetics Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics Genes Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/genetics Operon Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial Amidophosphoribosyltransferase Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Walker J E
Gay N J
Saraste M
Eberle A N
References (62)
62 references, click to expand
  1. Organization of unc gene cluster of Escherichia coli coding for proton-translocating ATPase of oxidative phosphorylation.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7005-9 PMID: 6261234
  2. The atp operon: nucleotide sequence of the genes for the gamma, beta, and epsilon subunits of Escherichia coli ATP synthase.
    Nucleic Acids Res. 1981 Oct 24;9(20):5287-96 PMID: 6272217
  3. Similar amino acid sequences: chance or common ancestry?
    Science. 1981 Oct 9;214(4517):149-59 PMID: 7280687
  4. The atp operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the delta subunit of Escherichia coli ATP-synthase.
    Nucleic Acids Res. 1981 Aug 25;9(16):3919-26 PMID: 6272190
  5. Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12.
    Nucleic Acids Res. 1981 Sep 25;9(18):4669-76 PMID: 6117826
  6. The atp operon: nucleotide sequence of the region encoding the alpha-subunit of Escherichia coli ATP-synthase.
    Nucleic Acids Res. 1981 May 11;9(9):2187-94 PMID: 6272228
  7. Nucleotide sequence of the gene coding for the delta subunit of proton translocating ATPase of Escherichia coli.
    Biochem Biophys Res Commun. 1981 Sep 16;102(1):172-9 PMID: 6458296
  8. Promoters in the E. coli replication origin.
    Nature. 1981 Nov 26;294(5839):376-8 PMID: 7031477
  9. Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase.
    J Biol Chem. 1982 Apr 10;257(7):3525-31 PMID: 6277938
  10. Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site, and trace metal analysis.
    J Biol Chem. 1982 Apr 10;257(7):3532-6 PMID: 7037784
  11. The genes for the eight subunits of the membrane bound ATP synthase of Escherichia coli.
    Mol Gen Genet. 1981;183(3):463-72 PMID: 6460910
  12. The nucleotide sequence of the atp genes coding for the F0 subunits a, b, c and the F1 subunit delta of the membrane bound ATP synthase of Escherichia coli.
    Mol Gen Genet. 1981;184(1):33-9 PMID: 6278247
  13. Codon preference and its use in identifying protein coding regions in long DNA sequences.
    Nucleic Acids Res. 1982 Jan 11;10(1):141-56 PMID: 7063399
  14. The complete nucleotide sequence of the tryptophan operon of Escherichia coli.
    Nucleic Acids Res. 1981 Dec 21;9(24):6647-68 PMID: 7038627
  15. Solid-phase sequence analysis of polypeptides eluted from polyacrylamide gels. An aid to interpretation of DNA sequences exemplified by the Escherichia coli unc operon and bacteriophage lambda.
    Eur J Biochem. 1982 Apr 1;123(2):253-60 PMID: 6210528
  16. Unique insertion site of Tn7 in the E. coli chromosome.
    Nature. 1982 Jun 17;297(5867):601-3 PMID: 6283361
  17. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
    Nucleic Acids Res. 1982 May 11;10(9):2951-61 PMID: 7099970
  18. Nucleotide sequence of the genes for beta and epsilon subunits of proton-translocating ATPase from Escherichia coli.
    Biochem Biophys Res Commun. 1982 Apr 29;105(4):1257-64 PMID: 6285901
  19. Differential polypeptide synthesis of the proton-translocating ATPase of Escherichia coli.
    J Bacteriol. 1982 Sep;151(3):1363-71 PMID: 6213603
  20. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  21. Nucleotide sequence of the promoter region of the gene cluster for proton-translocating ATPase from Escherichia coli and identification of the active promotor.
    Biochem Biophys Res Commun. 1982 Jul 30;107(2):568-75 PMID: 6215041
  22. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  23. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  24. Promoters of the atp operon coding for the membrane-bound ATP synthase of Escherichia coli mapped by Tn10 insertion mutations.
    Mol Gen Genet. 1982;188(2):240-8 PMID: 6185823
  25. Rapid similarity searches of nucleic acid and protein data banks.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PMID: 6572363
  26. Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli.
    J Mol Appl Genet. 1983;2(1):83-99 PMID: 6221060
  27. Nucleotide sequence of bacteriophage lambda DNA.
    J Mol Biol. 1982 Dec 25;162(4):729-73 PMID: 6221115
  28. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis.
    Anal Biochem. 1983 Feb 15;129(1):216-23 PMID: 6305233
  29. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  30. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  31. Cloning and expression of uncI, the first gene of the unc operon of Escherichia coli.
    J Bacteriol. 1983 Sep;155(3):1265-70 PMID: 6224772
  32. Promoter for the unc operon of Escherichia coli.
    J Bacteriol. 1983 Sep;155(3):1271-8 PMID: 6193096
  33. The glutamine-utilizing site of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase.
    J Biol Chem. 1983 Sep 10;258(17):10582-5 PMID: 6411716
  34. Cloning of the Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase gene in Escherichia coli. Nucleotide sequence determination and properties of the plasmid-encoded enzyme.
    J Biol Chem. 1983 Sep 10;258(17):10586-93 PMID: 6411717
  35. Nucleotide sequence of Escherichia coli pabA and its evolutionary relationship to trp(G)D.
    J Mol Biol. 1983 Aug 15;168(3):451-68 PMID: 6350604
  36. RNA polymerase interactions with the upstream region of the E. coli tyrT promoter.
    Cell. 1983 Nov;35(1):265-73 PMID: 6194900
  37. Analysis of Escherichia coli ATP synthase subunits by DNA and protein sequencing.
    Methods Enzymol. 1983;97:195-218 PMID: 6318027
  38. Graphic methods to determine the function of nucleic acid sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521-38 PMID: 6364040
  39. Isolation and characterization of a glucosamine-requiring mutant of Escherichia coli K-12 defective in glucosamine-6-phosphate synthetase.
    J Bacteriol. 1971 Feb;105(2):455-66 PMID: 5541523
  40. The nature of the polypeptide chain termination signal.
    J Mol Biol. 1971 Jun 14;58(2):513-31 PMID: 4933415
  41. The amidotransferases.
    Adv Enzymol Relat Areas Mol Biol. 1973;39:91-183 PMID: 4355768
  42. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  43. Glutamine active site of formylglycinamide ribonucleotide amidotransferase. 2. Amino acid sequence of labeled peptides.
    Biochemistry. 1977 Mar 22;16(6):1070-6 PMID: 849408
  44. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  45. Anthranilate synthetase component II from Pseudomonas putida. Covalent structure and identification of the cysteine residue involved in catalysis.
    J Biol Chem. 1978 Jul 10;253(13):4659-68 PMID: 659439
  46. Nucleotide sequence of Escherichia coli K-12 replication origin.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):575-9 PMID: 370832
  47. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4 PMID: 370833
  48. Total synthesis of a tyrosine suppressor transfer RNA gene. XVII. Transcription, in vitro, of the synthetic gene and processing of the primary transcript to transfer RNA.
    J Biol Chem. 1979 Jul 10;254(13):5802-16 PMID: 109442
  49. Deoxyribonucleic acid and outer membrane: binding to outer membrane involves a specific protein.
    J Bacteriol. 1979 Oct;140(1):43-9 PMID: 227834
  50. Deoxyribonucleic acid and outer membrane: strains diploid for the oriC region show elevated levels of deoxyribonucleic acid-binding protein and evidence for specific binding of the oriC region to outer membrane.
    J Bacteriol. 1979 Oct;140(1):50-8 PMID: 227835
  51. Primary structure of Serratia marcescens anthranilate synthase component II.
    J Biol Chem. 1980 Feb 25;255(4):1451-7 PMID: 6986371
  52. A tRNATyr promoter with an altered in vitro response to ppgpp.
    J Mol Biol. 1980 Jul 25;141(1):91-7 PMID: 6159476
  53. Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene.
    J Bacteriol. 1984 Mar;157(3):772-8 PMID: 6321434
  54. Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli.
    J Bacteriol. 1984 Mar;157(3):909-17 PMID: 6365894
  55. Affinity of two different regions of the chromosome to the outer membrane of Escherichia coli.
    J Bacteriol. 1984 Mar;157(3):968-70 PMID: 6321451
  56. Conserved features of coordinately regulated E. coli promoters.
    Nucleic Acids Res. 1984 Mar 26;12(6):2605-18 PMID: 6369249
  57. DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4134-8 PMID: 6330744
  58. Sequence of the small subunit of yeast carbamyl phosphate synthetase and identification of its catalytic domain.
    J Biol Chem. 1984 Aug 10;259(15):9790-8 PMID: 6086650
  59. The unc operon. Nucleotide sequence, regulation and structure of ATP-synthase.
    Biochim Biophys Acta. 1984 Sep 6;768(2):164-200 PMID: 6206892
  60. Azaserine-reactive sulfhydryl group of 2-formamido-N-ribosylacetamide 5'-phosphate: L-glutamine amido-ligase (adenosine diphosphate). II. Degradation of azaserine-C-14-labeled enzyme.
    J Biol Chem. 1963 Jun;238:2178-85 PMID: 14025584
  61. Glucosamine metabolism. V. Enzymatic synthesis of glucosamine 6-phosphate.
    J Biol Chem. 1960 May;235:1265-73 PMID: 13827775
  62. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
    Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2240799
Published
1984-12-15
Pages
799-815
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144516
Subset
IM
Databases
GENBANK
X01631
External Links
PubMed source DOI: 10.1042/bj2240799 PMC Full Text View journal details
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