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PMID: 8606150 Published · ppublish English Journal Article

Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinelandii.

Journal of bacteriology ·Vol. 178 ·No. 7 ·1996-04-00 ·Pages 1793-9

Campos M, Martínez-Salazar JM, Lloret L, Moreno S, Núñez C, Espín G, Soberón-Chávez G

Abstract

Azotobacter vinelandii presents a differentiation process leading to the formation of desiccation-resistant cysts. Alginate, the exopolysaccharide produced by this bacterium, has been postulated to have a role in cyst formation. Here, we report the cloning and characterization of the A. vinelandii gene coding for the enzyme GDP-mannose dehydrogenase (algD), which is the key enzyme for alginate synthesis in Pseudomonas aeruginosa. This gene has a high degree of similarity with the algD gene from P. aeruginosa, and similar proteins seem to be involved in algD regulation in both bacteria. We show the existence of two mRNA start sites; one of these sites corresponds to a promoter transcribed by RNA polymerase containing a sigma E subunit. An A. vinelandii algD mutant which is completely impaired in alginate production and which is unable to form desiccation-resistant cells was constructed. The effects of NH4, NO3, and NaCl concentrations on algD transcription for three A. vinelandii strains producing different alginate levels were evaluated. We found a strict correlation between alginate production and algD transcription for the three strains studied; however, the effects on algD transcription under the conditions studied were different for each strain. The nitrogen source regulates algD expression in the wild-type strain.

MeSH Terms
Alginates/metabolism Amino Acid Sequence Azotobacter vinelandii/enzymology,genetics Base Sequence Carbohydrate Dehydrogenases/genetics Cloning, Molecular DNA, Bacterial/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Mutation RNA, Messenger/genetics Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Alginates DNA, Bacterial RNA, Messenger Carbohydrate Dehydrogenases GDPmannose dehydrogenase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Campos M
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, México.
Martínez-Salazar J M
Lloret L
Moreno S
Núñez C
Espín G
Soberón-Chávez G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.7.1793-1799.1996
Published
1996-04-00
Pages
1793-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177871
Subset
IM
Databases
GENBANK
U11240
External Links
PubMed source DOI: 10.1128/jb.178.7.1793-1799.1996 PMC Full Text View journal details
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