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PMID: 862248 Published · ppublish English Journal Article

Human antibody-dependent cell-mediated cytotoxicity against target cells infected with respiratory syncytial virus.

Clinical and experimental immunology ·Vol. 28 ·No. 1 ·1977-04-00 ·Pages 19-26

Scott R, de Landazuri MO, Gardner PS, Owen JJ

Abstract

A chromium release assay was established to study human antibody-dependent cell-mediated cytotoxicity (ADCC) of HEp 2 cells infected with respiratory syncytial (RS) virus. Human peripheral blood lymphocytes in the presence of specific antibody to RS virus caused in vitro lysis of RS virus infected target cells. ADCC was detected in sera of infants with RS virus infections and in specimens of colostrum. The ability of serum or colostrum to mediate the cytotoxic reaction appeared to be related to the level of specific IgG, or IgA antibody to RS virus, as detected by membrane fluorescence. Separation of effector cells by their glass adherence properties showed that the ability to produce cytotoxicity resided in non-adherent effector cells.

MeSH Terms
Antibodies, Viral Antigens, Viral Cells, Cultured Colostrum/immunology Cytotoxicity Tests, Immunologic Humans Immunity, Cellular Infant Lymphocytes/immunology Respiratory Syncytial Viruses/immunology Respiratory Tract Infections/immunology
Chemicals
Antibodies, Viral Antigens, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Scott R
de Landazuri M O
Gardner P S
Owen J J
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24 references, click to expand
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1977-04-00
Pages
19-26
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1540874
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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