Home LiteratureArticle Details
PMID: 8625740 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA representation of variegating heterochromatic P-element inserts in diploid and polytene tissues of Drosophila melanogaster.

Chromosoma ·Vol. 104 ·No. 7 ·1996-04-00 ·Pages 519-27

Wallrath LL, Guntur VP, Rosman LE, Elgin SC

Abstract

Position-effect variegation (PEV) is the mosaic expression of a euchromatic gene brought into juxtaposition with heterochromatin. Fourteen different transformed Drosophila melanogaster lines with variegating P-element inserts were used to examine the DNA levels of these transgenes. Insert sites include pericentric, telomeric and fourth chromosome regions. Southern blot analyses showed that the heterochromatic hsp26 transgenes are underrepresented 1.3- to 33-fold in polytene tissue relative to the endogenous euchromatic hsp26 gene. In contrast, the heterochromatic hsp26 transgenes are present in approximately the same copy number as the endogenous euchromatic hsp26 gene in diploid tissue. It appears unlikely that DNA loss could account for the lack of gene expression in diploid tissues seen with these examples of PEV.

MeSH Terms
ATP-Binding Cassette Transporters Animals Cell Line Chromatin/genetics DNA Transposable Elements/genetics Diploidy Drosophila Proteins Drosophila melanogaster/genetics,growth & development Euchromatin Eye Color/genetics Eye Proteins Female Gene Expression Regulation, Developmental Genes, Insect HSP70 Heat-Shock Proteins/genetics Heat-Shock Proteins/genetics Heterochromatin/genetics Insect Hormones/genetics Larva Male Mosaicism/genetics Mutagenesis, Insertional Salivary Glands/ultrastructure Telomere
Chemicals
ATP-Binding Cassette Transporters Chromatin DNA Transposable Elements Drosophila Proteins Euchromatin Eye Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins Heterochromatin Hsp26 protein, Drosophila Insect Hormones w protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wallrath L L
Department of Biology, Washington University, St. Louis, MO 63130, USA.
Guntur V P
Rosman L E
Elgin S C
References (51)
51 references, click to expand
  1. Mapping simple repeated DNA sequences in heterochromatin of Drosophila melanogaster.
    Genetics. 1993 Aug;134(4):1149-74 PMID: 8375654
  2. The Drosophila salivary gland chromocenter contains highly polytenized subdomains of mitotic heterochromatin.
    Genetics. 1995 Feb;139(2):659-70 PMID: 7713423
  3. Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage.
    Genes Dev. 1993 Jul;7(7A):1133-45 PMID: 8319906
  4. DNA of Drosophila chromosomes.
    Annu Rev Genet. 1973;7:177-204 PMID: 4593302
  5. Position effect variegation and genomic instability.
    Cold Spring Harb Symp Quant Biol. 1993;58:585-96 PMID: 7956073
  6. Reptitive DNA sequences in drosophila.
    Chromosoma. 1971;33(3):319-44 PMID: 5088497
  7. Chromatin diminution in nematode development.
    Trends Genet. 1992 Dec;8(12):427-32 PMID: 1492368
  8. The Y chromosome of Drosophila melanogaster contains a distinctive subclass of Het-A-related repeats.
    Genetics. 1993 Jun;134(2):531-43 PMID: 8392014
  9. Dosage compensation in Drosophila.
    Annu Rev Genet. 1994;28:491-521 PMID: 7893138
  10. Overcoming telomeric silencing: a trans-activator competes to establish gene expression in a cell cycle-dependent way.
    Genes Dev. 1994 May 15;8(10):1133-46 PMID: 7926719
  11. Raising large quantities of Drosophila for biochemical experiments.
    Methods Cell Biol. 1994;44:99-108 PMID: 7707979
  12. Transformation of white locus DNA in drosophila: dosage compensation, zeste interaction, and position effects.
    Cell. 1984 Feb;36(2):469-81 PMID: 6319027
  13. Germ line-restricted, highly repeated DNA sequences and their chromosomal localization in a Japanese hagfish (Eptatretus okinoseanus).
    Chromosoma. 1993 Feb;102(3):163-73 PMID: 8458254
  14. HeT DNA: a family of mosaic repeated sequences specific for heterochromatin in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1990 Oct;87(20):7998-8002 PMID: 2122452
  15. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  16. The formation of polytene chromosomes during macronuclear development of the hypotrichous ciliate Stylonychia mytilus.
    Chromosoma. 1981;82(2):309-14 PMID: 6785046
  17. Cis-effects of heterochromatin on heterochromatic and euchromatic gene activity in Drosophila melanogaster.
    Genetics. 1995 Jul;140(3):1033-45 PMID: 7672575
  18. The effects of chromosome rearrangements on the expression of heterochromatic genes in chromosome 2L of Drosophila melanogaster.
    Genetics. 1990 May;125(1):141-54 PMID: 2111264
  19. Telomere-associated chromosome breakage in fission yeast results in variegated expression of adjacent genes.
    EMBO J. 1994 Aug 15;13(16):3801-11 PMID: 8070408
  20. Promoter sequence containing (CT)n.(GA)n repeats is critical for the formation of the DNase I hypersensitive sites in the Drosophila hsp26 gene.
    J Mol Biol. 1992 Jun 20;225(4):985-98 PMID: 1377279
  21. Position effect variegation in Drosophila is associated with an altered chromatin structure.
    Genes Dev. 1995 May 15;9(10):1263-77 PMID: 7758950
  22. The phenomenon of position effect.
    Adv Genet. 1950;3:73-115 PMID: 15425389
  23. Variegated expression of the Sgs-4 locus in Drosophila melanogaster.
    Chromosoma. 1986;94(3):205-16 PMID: 3095057
  24. Unusual properties of genomic DNA molecules spanning the euchromatic-heterochromatic junction of a Drosophila minichromosome.
    Nucleic Acids Res. 1994 Nov 25;22(23):5068-75 PMID: 7800501
  25. Heterochromatin and gene expression in Drosophila.
    Annu Rev Genet. 1995;29:577-605 PMID: 8825487
  26. Sixty years of mystery.
    Genetics. 1990 Dec;126(4):779-84 PMID: 2127578
  27. Studies on the mechanism of heterochromatic position effect at the rosy locus of Drosophila melanogaster.
    Genetics. 1984 Nov;108(3):603-15 PMID: 6149972
  28. A possible case of position effect of DNA replication in Drosophila melanogaster.
    Genetics. 1972 Dec;72(4):607-14 PMID: 4631595
  29. Cytogenetic and molecular aspects of position effect variegation in Drosophila. III. Continuous and discontinuous compaction of chromosomal material as a result of position effect variegation.
    Chromosoma. 1991 Aug;100(7):453-66 PMID: 1914664
  30. Position-effect variegation after 60 years.
    Trends Genet. 1990 Dec;6(12):422-6 PMID: 2087785
  31. Evidence for intrinsic differences in the formation of chromatin domains in Drosophila melanogaster.
    Genetics. 1992 Dec;132(4):1063-9 PMID: 1459427
  32. Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster.
    J Mol Biol. 1983 Jul 25;168(1):17-33 PMID: 6410077
  33. Non replicating DNA in Drosophila.
    Genetics. 1969;61(1):Suppl:227-38 PMID: 5345382
  34. Studies of He-T DNA sequences in the pericentric regions of Drosophila chromosomes.
    Chromosoma. 1989 Jan;97(4):261-71 PMID: 2565198
  35. Genomic distribution of copia-like transposable elements in somatic tissues and during development of Drosophila melanogaster.
    Chromosoma. 1989 Dec;98(6):402-10 PMID: 2560696
  36. A stable genomic source of P element transposase in Drosophila melanogaster.
    Genetics. 1988 Mar;118(3):461-70 PMID: 2835286
  37. Position-effect variegation and the new biology of heterochromatin.
    Curr Opin Genet Dev. 1994 Apr;4(2):281-91 PMID: 8032206
  38. Analysis of subtelomeric heterochromatin in the Drosophila minichromosome Dp1187 by single P element insertional mutagenesis.
    Genetics. 1992 Nov;132(3):737-53 PMID: 1334894
  39. Replication forks are not found in a Drosophila minichromosome demonstrating a gradient of polytenization.
    Chromosoma. 1992 Dec;102(1):15-9 PMID: 1291225
  40. Cytogenetic and molecular aspects of position effect variegation in Drosophila melanogaster. II. Peculiarities of morphology and genetic activity of the 2B region in the T(1;2)dorvar7 chromosome in males.
    Chromosoma. 1988;96(3):255-61 PMID: 3129255
  41. Reduced DNA polytenization of a minichromosome region undergoing position-effect variegation in Drosophila.
    Cell. 1990 Oct 5;63(1):97-107 PMID: 2208283
  42. Cytogenetic and molecular aspects of position-effect variegation in Drosophila melanogaster. V. Heterochromatin-associated protein HP1 appears in euchromatic chromosomal regions that are inactivated as a result of position-effect variegation.
    Chromosoma. 1993 Sep;102(8):583-90 PMID: 8243169
  43. Position-effect variegation and chromosome structure of a heat shock puff in Drosophila.
    Chromosoma. 1981;83(3):381-93 PMID: 6168446
  44. Variegation in Drosophila and the Inert Chromosome Regions.
    Proc Natl Acad Sci U S A. 1936 Jan;22(1):27-33 PMID: 16588038
  45. Chromosomal structure is altered by mutations that suppress or enhance position effect variegation.
    Chromosoma. 1990 Oct;99(6):391-400 PMID: 2125551
  46. Chromosome structure at interfaces between major chromatin types: alpha- and beta-heterochromatin.
    Bioessays. 1990 Jan;12(1):1-6 PMID: 2108664
  47. RAP1 and telomere structure regulate telomere position effects in Saccharomyces cerevisiae.
    Genes Dev. 1993 Jul;7(7A):1146-59 PMID: 8319907
  48. Changed pattern of transcription and replication in polytene chromosomes of Drosophila melanogaster resulting from eu-heterochromatin rearrangement.
    Chromosoma. 1974 Mar 14;45(2):173-91 PMID: 4210095
  49. Characterization of Drosophila heterochromatin. II. C- and N-banding.
    Chromosoma. 1976 Sep 24;57(4):377-86 PMID: 63359
  50. Cytogenetic and molecular aspects of position effect variegation in Drosophila melanogaster. IV. Underreplication of chromosomal material as a result of gene inactivation.
    Chromosoma. 1991 Oct;101(1):55-61 PMID: 1769274
  51. Analysis of a whole chromosome in Drosophila.
    Cold Spring Harb Symp Quant Biol. 1974;38:581-9 PMID: 4208793
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00352116
Published
1996-04-00
Pages
519-27
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM14516 · United States
NICHD NIH HHS · HD23844 · United States
External Links
PubMed source DOI: 10.1007/BF00352116 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2