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PMID: 8626318 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Site-specific insertion of IS1301 and distribution in Neisseria meningitidis strains.

Journal of bacteriology ·Vol. 178 ·No. 9 ·1996-05-00 ·Pages 2527-32

Hilse R, Hammerschmidt S, Bautsch W, Frosch M

Abstract

The insertion element IS1301 has been shown to mediate capsule phase variation in Neisseria meningitidis found in N. serogroup B by reversible insertional inactivation of the siaA gene. We have determined the target site specificity of this element by cloning and sequencing the insertion sites of 12 identical IS1301 copies found in N. meningitidis B1940. A target consensus core of 5'-AYTAG-3' was identified, with the central TA being duplicated following insertion. Additional features around the target sites, including extended palindromic symmetry, stem-loop formation, and the high incidence of AT tracts, indicate that other factors, such as DNA secondary structure, are involved in target recognition. The left inverted repeat of an IS1016-like element acts as a hot spot for insertion, with one insertion element combination located upstream of their gene. According to further sequence analysis, we were able to place IS1301 in the IS5 subgroup within the IS4 family of elements. A survey of 135 Neisseria strains indicated the presence of IS1301 in 27.9 to 33.3% of N. meningitides serogroup B, C, and W135 strains and in 86.7% of serogroup Y strains. IS1301 did not occur in serogroup A strains, in Neisseria gonorrhoeae, or in apathogenic Neisseria spp.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular Consensus Sequence/genetics Cytotoxins DNA Transposable Elements/genetics DNA, Bacterial/chemistry,genetics Membrane Proteins Molecular Sequence Data Neisseria meningitidis/genetics Nucleic Acid Conformation Restriction Mapping Sequence Analysis, DNA
Chemicals
Bacterial Proteins Cytotoxins DNA Transposable Elements DNA, Bacterial Membrane Proteins frpC protein, Neisseria meningitidis
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hilse R
Institut für Medizinische Mikrobiologie, Medizinische Hochschule, Hannover, Germany.
Hammerschmidt S
Bautsch W
Frosch M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.9.2527-2532.1996
Published
1996-05-00
Pages
2527-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177975
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.178.9.2527-2532.1996 PMC Full Text View journal details
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