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PMID: 8627748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nuclear trafficking of influenza virus ribonuleoproteins in heterokaryons.

Journal of virology ·Vol. 70 ·No. 5 ·1996-05-00 ·Pages 2743-56

Whittaker G, Bui M, Helenius A

Abstract

The influenza virus nucleoprotein (NP), matrix protein (M1), and ribonucleoproteins (vRNPs) undergo regulated nuclear import and export during infection. Their trafficking was analyzed by using interspecies heterokaryons containing nuclei from infected and uninfected cells. Under normal conditions, it was demonstrated that the vRNPs which were assembled in the nucleus and transported to the cytosol were prevented from reimport into the nucleus. To be import competent, they must first assemble into virions and enter by the endosomal entry pathway. In influenza virus mutant ts51, in which M1 is defective, direct reimport took place but was inhibited by heterologous expression of wild-type M1. These data confirm M1's role as the inhibitor of premature nuclear import and as the main regulator of nuclear transport of vRNPs. In addition to this vRNP shuttling, M1 also shuttled between the nucleus and the cytoplasm in ts51-infected cells. When NP was expressed in the absence of virus infection, it was also found to be a shuttling protein.

MeSH Terms
Animals Cattle Cell Fusion Cell Line Cell Nucleus/physiology,ultrastructure,virology Cytosol/physiology Dogs Fluorescent Antibody Technique HeLa Cells Humans Influenza A virus/genetics,physiology,ultrastructure Microscopy, Confocal Ribonucleoproteins/metabolism Viral Matrix Proteins/metabolism Virion/physiology Virus Replication
Chemicals
Ribonucleoproteins Viral Matrix Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Whittaker G
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA.
Bui M
Helenius A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.5.2743-2756.1996
Published
1996-05-00
Pages
2743-56
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190131
Subset
IM
Grants
NIAID NIH HHS · AI 18599 · United States
NIGMS NIH HHS · GM 07205 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.5.2743-2756.1996 PMC Full Text View journal details
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