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PMID: 1985199 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transport of incoming influenza virus nucleocapsids into the nucleus.

Journal of virology ·Vol. 65 ·No. 1 ·1991-01-00 ·Pages 232-44

Martin K, Helenius A

Abstract

Upon penetration of the influenza virus nucleocapsid into the host cell cytoplasm, the viral RNA and associated proteins are transported to the nucleus, where viral transcription and replication occur. By using quantitative confocal microscopy, we have found that over half of cell-associated nucleoprotein (NP) entered the nucleus with a half time of 10 min after penetration into CHO cells. Microinjection and immunoelectron microscopy experiments indicated that the NP entered the nucleus through the nuclear pore as part of an intact ribonucleoprotein (RNP) structure and that its transport was an active process. Transport of the incoming RNPs into the nucleus was not dependent on an intact microfilament, microtubule, or intermediate filament network. Subsequent to penetration, the matrix (M1) protein appeared to dissociate from the RNP structure and to enter the nucleus independently of the RNP. We found that 50% of penetrated M1 entered the nucleus with a half time of 25 min after penetration into CHO cells. Nuclear transport of M1 appeared to occur by passive diffusion. Entry of incoming M1 into the nucleus was not a prerequisite for infection.

MeSH Terms
Animals Capsid/metabolism Cell Line Cell Nucleus/metabolism Cell Transformation, Viral Cysteine/metabolism Fluorescent Antibody Technique Influenza A virus/genetics,physiology,ultrastructure Kinetics Methionine/metabolism Microscopy, Immunoelectron Viral Core Proteins/metabolism Viral Proteins/biosynthesis,metabolism Viral Structural Proteins/metabolism
Chemicals
Viral Core Proteins Viral Proteins Viral Structural Proteins Methionine Cysteine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Martin K
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510.
Helenius A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.1.232-244.1991
Published
1991-01-00
Pages
232-44
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240510
Subset
IM
Grants
NIAID NIH HHS · AI18599 · United States
External Links
PubMed source DOI: 10.1128/JVI.65.1.232-244.1991 PMC Full Text View journal details
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