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PMID: 8628220 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential expression of genes in the vir regulon of Streptococcus pyogenes is controlled by transcription termination.

Molecular & general genetics : MGG ·Vol. 250 ·No. 2 ·1996-02-05 ·Pages 207-13

Pritchard KH, Cleary PP

Abstract

Streptococcal C5a peptidase (SCP), encoded by scpA in Streptococcus pyogenes, is a surface molecule which is able to cleave and inactivate the chemotactic factor C5a. The scpA gene is part of the vir regulon and subject to positive regulation by the Mga protein. It is down-regulated compared to another Mga-activated gene, emm. A chloramphenicol acetyltransferase (CAT) reporter gene was used to measure scpA promoter activity. Previous work had shown that when a large portion of the scpA promoter region was deleted, expression of CAT increased relative to the wild-type. This deleted region was found to contain an inverted repeat. In this study we show that the inverted repeat in the leader mRNA is the site of transcription termination, which down-regulates expression of scpA. This is a novel mechanism for regulation of gene expression in S. pyogenes. A specific deletion of the inverted repeat in the scpA promoter-CAT reporter construct was made using inverse PCR. Expression was measured from single-copy chromosomal integrants. When the inverted repeat was deleted, expression increased. Furthermore, Northern hybridization confirmed the existence of a truncated transcript, consistent with a transcription termination mechanism.

MeSH Terms
Adhesins, Bacterial Bacterial Proteins/pharmacology Base Sequence Blotting, Northern Cloning, Molecular Down-Regulation Endopeptidases/chemistry,genetics Gene Expression Regulation, Bacterial Genes, Reporter Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic/genetics RNA/genetics,metabolism Repetitive Sequences, Nucleic Acid/genetics Sequence Analysis Sequence Deletion/genetics Streptococcus pyogenes/enzymology,genetics,pathogenicity Terminator Regions, Genetic/genetics Transcription Factors/pharmacology Transcription, Genetic Virulence/genetics
Chemicals
Adhesins, Bacterial Bacterial Proteins Transcription Factors mry protein, Streptococcus pyogenes RNA Endopeptidases C5a peptidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pritchard K H
Department of Microbiology, University of Minnesota, Minneapolis 55455, USA.
Cleary P P
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28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02174180
Published
1996-02-05
Pages
207-13
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI 16722 · United States
NCI NIH HHS · CA 09138-20 · United States
External Links
PubMed source DOI: 10.1007/BF02174180
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