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PMID: 8636054 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of a pdxJ point mutation that bypasses the requirement for the PdxH oxidase in pyridoxal 5' -phosphate coenzyme biosynthesis in Escherichia coli K-12.

Journal of bacteriology ·Vol. 178 ·No. 8 ·1996-04-00 ·Pages 2445-9

Man TK, Zhao G, Winkler ME

Abstract

We isolated 26 suppressor mutations that allowed growth of a delta pdxH::omega null mutant in the absence of pyridoxal. Each suppressor mapped to pdxJ, and the eight suppressors sequenced contained the same glycine-to-serine change in the PdxJ polypeptide. This bypass suppression suggests that PdxJ may participate in formation of the pyridine ring of pyridoxine 5'-phosphate.

MeSH Terms
Bacterial Proteins/genetics Escherichia coli/enzymology,genetics Escherichia coli Proteins Ligases Models, Biological Mutagenesis, Insertional Point Mutation Pyridoxal Phosphate/analogs & derivatives,biosynthesis,metabolism Pyridoxaminephosphate Oxidase/genetics,metabolism Sequence Deletion Suppression, Genetic
Chemicals
Bacterial Proteins Escherichia coli Proteins PdxJ protein, E coli Pyridoxal Phosphate Pyridoxaminephosphate Oxidase Ligases pyridoxine 5-phosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Man T K
Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School 77030-1501, USA.
Zhao G
Winkler M E
References (27)
27 references, click to expand
  1. Cloning and characterization of the Escherichia coli hemN gene encoding the oxygen-independent coproporphyrinogen III oxidase.
    J Bacteriol. 1995 Jun;177(11):3326-31 PMID: 7768836
  2. Characterization of the complex pdxH-tyrS operon of Escherichia coli K-12 and pleiotropic phenotypes caused by pdxH insertion mutations.
    J Bacteriol. 1992 Oct;174(19):6033-45 PMID: 1356963
  3. Growth response to 4-hydroxy-L-threonine of Escherichia coli mutants blocked in vitamin B6 biosynthesis.
    FEBS Lett. 1993 Mar 1;318(2):125-8 PMID: 8440369
  4. Kinetic limitation and cellular amount of pyridoxine (pyridoxamine) 5'-phosphate oxidase of Escherichia coli K-12.
    J Bacteriol. 1995 Feb;177(4):883-91 PMID: 7860596
  5. Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.
    J Bacteriol. 1985 Mar;161(3):1219-21 PMID: 2982787
  6. The genes required for heme synthesis in Salmonella typhimurium include those encoding alternative functions for aerobic and anaerobic coproporphyrinogen oxidation.
    J Bacteriol. 1992 Jun;174(12):3953-63 PMID: 1317844
  7. Pyridoxamine phosphate-oxidase and pyridoxal phosphate-phosphatase activities in Escherichia coli.
    Biochem J. 1961 Feb;78:364-72 PMID: 13778735
  8. Purification and properties of vitamin B6 kinase from Escherichia coli B.
    Biochemistry. 1970 Oct 13;9(21):4057-64 PMID: 4917899
  9. Purification and characterization of the pyridoxol-5'-phosphate:oxygen oxidoreductase (deaminating) from Escherichia coli.
    Biochim Biophys Acta. 1995 Mar 15;1247(2):265-71 PMID: 7696318
  10. Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.
    J Bacteriol. 1992 Mar;174(5):1544-53 PMID: 1537799
  11. Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12.
    Mol Microbiol. 1994 Jul;13(1):35-49 PMID: 7984093
  12. Metabolic relationships between pyridoxine (vitamin B6) and serine biosynthesis in Escherichia coli K-12.
    J Bacteriol. 1990 Nov;172(11):6518-28 PMID: 2121717
  13. Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette.
    J Bacteriol. 1987 Mar;169(3):1061-70 PMID: 3029016
  14. 4-Phospho-hydroxy-L-threonine is an obligatory intermediate in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12.
    FEMS Microbiol Lett. 1996 Jan 15;135(2-3):275-80 PMID: 8595869
  15. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  16. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  17. Biochemical characterization of gapB-encoded erythrose 4-phosphate dehydrogenase of Escherichia coli K-12 and its possible role in pyridoxal 5'-phosphate biosynthesis.
    J Bacteriol. 1995 May;177(10):2804-12 PMID: 7751290
  18. Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12.
    J Bacteriol. 1989 Sep;171(9):4767-77 PMID: 2670894
  19. An Escherichia coli K-12 tktA tktB mutant deficient in transketolase activity requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth.
    J Bacteriol. 1994 Oct;176(19):6134-8 PMID: 7928977
  20. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  21. Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations.
    J Bacteriol. 1992 Mar;174(5):1554-67 PMID: 1537800
  22. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
  23. Synthesis of Pyridoxine by a Pyridoxal Auxotroph of Escherichia coli.
    J Bacteriol. 1966 Aug;92(2):333-7 PMID: 16562115
  24. Cloning, DNA sequence, and complementation analysis of the Salmonella typhimurium hemN gene encoding a putative oxygen-independent coproporphyrinogen III oxidase.
    J Bacteriol. 1994 Jun;176(11):3196-203 PMID: 8195073
  25. Structural features of the hisT operon of Escherichia coli K-12.
    Nucleic Acids Res. 1985 Jul 25;13(14):5297-315 PMID: 2991861
  26. Indirect suppression of recB and recC mutations by exonuclease I deficiency.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1366-70 PMID: 4556461
  27. Stable isotope studies on the biosynthesis of the thiazole moiety of thiamin in Escherichia coli.
    Biochemistry. 1978 Sep 5;17(18):3833-40 PMID: 359046
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.8.2445-2449.1996
Published
1996-04-00
Pages
2445-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177961
Subset
IM
Grants
NIGMS NIH HHS · R01-GM 37561 · United States
External Links
PubMed source DOI: 10.1128/jb.178.8.2445-2449.1996 PMC Full Text View journal details
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