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PMID: 8636239 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Heterophilic interactions of DM-GRASP: GRASP-NgCAM interactions involved in neurite extension.

The Journal of cell biology ·Vol. 133 ·No. 3 ·1996-05-00 ·Pages 657-66

DeBernardo AP, Chang S

Abstract

DM-GRASP is an immunoglobulin superfamily cell adhesion molecule that is expressed in both the developing nervous and immune system. Specific populations of neurons respond to DM-GRASP substrates appears to require homophilic interactions between DM-GRASP molecules. We were interested in determining whether DM-GRASP interacts heterophilically with other ligands as well. We have found that eleven proteins from embryonic chick brain membranes consistently bind to and elute from a DM-GRASP-Sepharose affinity column. One of these proteins is DM-GRASP itself, consistent with its known homophilic binding. Another protein, at 130 kD, is immunoreactive with monoclonal antibodies to NgCAM. Other neural cell adhesion molecules were not detected in the eluate. The DM-GRASP-Sepharose eluate also contains a potent neurite stimulating activity, which cannot be accounted for by either DM-GRASP or NgCAM. To investigate the interaction of DM-GRASP and NgCAM, antibodies against DM-GRASP were added to neuronal cultures extending neurites on an NgCAM substrate. The presence of antibodies to DM-GRASP decreased neurite extension on laminin, suggesting that the antibody is not toxic or generally inhibiting motility. We present two possible models for the DM-GRASP-NgCAM association and a hypothesis for neural cell adhesion function that features the dimerization of cell adhesion molecules.

MeSH Terms
Activated-Leukocyte Cell Adhesion Molecule Animals Antibodies, Monoclonal Axons/physiology Blotting, Western Cell Adhesion/physiology Cell Adhesion Molecules, Neuronal/immunology,isolation & purification,pharmacology Cells, Cultured/cytology,drug effects Chick Embryo Chromatography, Affinity Extracellular Matrix Proteins/analysis,immunology,pharmacology Ganglia, Spinal/cytology Nerve Tissue Proteins/analysis,immunology,pharmacology Neurites/drug effects,physiology Neurons/cytology,ultrastructure Sepharose
Chemicals
Activated-Leukocyte Cell Adhesion Molecule Antibodies, Monoclonal Cell Adhesion Molecules, Neuronal Extracellular Matrix Proteins Nerve Tissue Proteins Sepharose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DeBernardo A P
Department of Neuroscience, School of Medicine, University of Pennsylvania, Philidelphia 19104, USA.
Chang S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.133.3.657
Published
1996-05-00
Pages
657-66
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120810
Subset
IM
Grants
NINDS NIH HHS · NS 26519 · United States
External Links
PubMed source DOI: 10.1083/jcb.133.3.657 PMC Full Text View journal details
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