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PMID: 8643650 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

E2F1 and E1A(12S) have a homologous activation domain regulated by RB and CBP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 4 ·1996-02-20 ·Pages 1439-42

Trouche D, Kouzarides T

Abstract

The E2F1 transcription factor has a well-characterized activation domain at its C terminus and the E1A protein has a recently defined activation domain at its N terminus. Here we show that these activation domains are highly related in sequence. The sequence homology reflects, at least partly, the conservation of common binding sites for the RB and CBP/p300 proteins, which are preserved in the same relative order along E2F1 and E1A. Furthermore, the interaction of RB and CBP with these two activation domains results in the same functional consequences: RB represses both activation domains, whereas CBP stimulates them. We conclude that the activation domains of E1A(12s) and E2F1 belong to a novel functional class, characterized by specific protein binding sites. The implication of this conservation with respect to E1A-induced stimulation of E2F activity is discussed.

MeSH Terms
Adenovirus E1A Proteins/chemistry,metabolism Amino Acid Sequence Binding Sites Bone Neoplasms/pathology CREB-Binding Protein Carrier Proteins Cell Cycle Proteins DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor Humans Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins/metabolism Osteosarcoma/pathology Promoter Regions, Genetic Protein Binding Recombinant Fusion Proteins/biosynthesis Retinoblastoma Protein/metabolism Retinoblastoma-Binding Protein 1 Sequence Homology, Amino Acid Structure-Activity Relationship Trans-Activators Transcription Factor DP1 Transcription Factors/chemistry,metabolism Tumor Cells, Cultured
Chemicals
Adenovirus E1A Proteins Carrier Proteins Cell Cycle Proteins DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human Nuclear Proteins Recombinant Fusion Proteins Retinoblastoma Protein Retinoblastoma-Binding Protein 1 Trans-Activators Transcription Factor DP1 Transcription Factors CREB-Binding Protein CREBBP protein, human
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Trouche D
Wellcome/Cancer Research Campaign Institute, University of Cambridge, United Kingdom.
Kouzarides T
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.4.1439
Published
1996-02-20
Pages
1439-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC39957
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1073/pnas.93.4.1439 PMC Full Text View journal details
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