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PMID: 8035489 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Complementary functions of E1a conserved region 1 cooperate with conserved region 3 to activate adenovirus serotype 5 early promoters.

Journal of virology ·Vol. 68 ·No. 8 ·1994-08-00 ·Pages 4910-20

Wong HK, Ziff EB

Abstract

The amino-terminal region of the adenovirus type 5 E1a protein including conserved regions (CRs) 1 and 2 binds the 105-kDa retinoblastoma protein and a second, 300-kDa, cellular protein. We show that mutant viruses with deletions of CR1 which release the binding of either p105 or p300 still activate early promoters and infect cells productively. However, mutations which disrupt binding of both proteins disrupt early promoter activity and block the viral life cycle. Ela CR3, which has an established role in early promoter activation, can act in trans to the amino-terminal functions. This suggests that the amino terminus provides distinct, redundant functions related to p300 and Rb binding that synergize with CR3 to transactivate early genes.

MeSH Terms
3T3 Cells Adenoviridae/classification,genetics,growth & development Adenovirus E1A Proteins/genetics,metabolism Animals Cell Line Conserved Sequence Diploidy E1A-Associated p300 Protein Fibroblasts/cytology Gene Expression Regulation, Viral Genetic Complementation Test Humans Mice Mutation Nuclear Proteins/metabolism Promoter Regions, Genetic Retinoblastoma Protein/genetics,metabolism Sequence Deletion Serotyping Trans-Activators Transcription Factors/metabolism Transcription, Genetic Virus Replication
Chemicals
Adenovirus E1A Proteins Nuclear Proteins Retinoblastoma Protein Trans-Activators Transcription Factors E1A-Associated p300 Protein Ep300 protein, mouse
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wong H K
Howard Hughes Medical Institute, Department of Biochemistry, New York University Medical Center, New York 10016.
Ziff E B
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.8.4910-4920.1994
Published
1994-08-00
Pages
4910-20
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236431
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.8.4910-4920.1994 PMC Full Text 免费全文 View journal details
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