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PMID: 1534143 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA-binding properties of the E1A-associated 300-kilodalton protein.

Molecular and cellular biology ·Vol. 12 ·No. 6 ·1992-06-00 ·Pages 2826-36

Rikitake Y, Moran E

Abstract

One of the major E1A-associated cellular proteins is a 300-kDa product (p300) that binds to the N-terminal region of the E1A products. The p300 binding site is distinct from sequences involved in binding the retinoblastoma product and other E1A-associated cellular products such as p60-cyclin A and p107. p300 binding to E1A is linked genetically to the enhancer repression function of E1A and the other E1A-mediated gene-regulating functions as well as to the transforming functions of E1A. However, the biochemical properties of p300 have not yet been characterized. We report here that p300 has an intrinsic DNA-binding activity and shows a preferential affinity for specific DNA sequences. The sequences selectively bound by p300 are related to those of a series of enhancer elements that are recognized by NF-kappa B. The direct physical interaction of p300 with enhancer elements provides a biochemical basis for the genetic evidence linking the E1A-mediated enhancer repression function with the p300-binding activity of E1A.

MeSH Terms
Adenovirus Early Proteins Base Sequence Cross-Linking Reagents DNA-Binding Proteins/isolation & purification,metabolism Enhancer Elements, Genetic HeLa Cells Humans Macromolecular Substances Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides/chemistry Oncogene Proteins, Viral/isolation & purification,metabolism Protein Binding Transcription Factors/metabolism Ultraviolet Rays
Chemicals
Adenovirus Early Proteins Cross-Linking Reagents DNA-Binding Proteins Macromolecular Substances Oligodeoxyribonucleotides Oncogene Proteins, Viral Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rikitake Y
Cold Spring Harbor Laboratory, New York 11724.
Moran E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.6.2826-2836.1992
Published
1992-06-00
Pages
2826-36
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364477
Subset
IM
Grants
NCI NIH HHS · CA-53592 · United States
NCI NIH HHS · CA-55330 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.6.2826-2836.1992 PMC Full Text View journal details
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