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PMID: 8647398 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The Drosophila meiotic recombination gene mei-9 encodes a homologue of the yeast excision repair protein Rad1.

Genetics ·Vol. 141 ·No. 2 ·1995-10-00 ·Pages 619-27

Sekelsky JJ, McKim KS, Chin GM, Hawley RS

Abstract

Meiotic recombination and DNA repair are mediated by overlapping sets of genes. In the yeast Saccharomyces cerevisiae, many genes required to repair DNA double-strand breaks are also required for meiotic recombination. In contrast, mutations in genes required for nucleotide excision repair (NER) have no detectable effects on meiotic recombination in S. cerevisiae. The Drosophila melanogaster mei-9 gene is unique among known recombination genes in that it is required for both meiotic recombination and NER. We have analyzed the mei-9 gene at the molecular level and found that it encodes a homologue of the S. cerevisiae excision repair protein Rad1, the probable homologue of mammalian XPF/ERCC4. Hence, the predominant process of meiotic recombination in Drosophila proceeds through a pathway that is at least partially distinct from that of S. cerevisiae, in that it requires an NER protein. The biochemical properties of the Rad1 protein allow us to explain the observation that mei-9 mutants suppress reciprocal exchange without suppressing the frequency of gene conversion.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins/genetics DNA Primers DNA Repair/genetics DNA Repair Enzymes DNA Transposable Elements DNA-Binding Proteins Drosophila Proteins Drosophila melanogaster/drug effects,genetics Endonucleases/genetics Fungal Proteins/genetics Genes, Fungal Genes, Insect Meiosis Methyl Methanesulfonate/pharmacology Molecular Sequence Data Mutagenesis Nuclear Proteins Polymerase Chain Reaction Protein Biosynthesis Proteins/chemistry,genetics Receptors, Steroid/metabolism Recombination, Genetic/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
Carrier Proteins DNA Primers DNA Transposable Elements DNA-Binding Proteins Drosophila Proteins Fungal Proteins MEI-9 protein, Drosophila Nuclear Proteins Proteins Receptors, Steroid Saccharomyces cerevisiae Proteins Methyl Methanesulfonate Endonucleases RAD1 protein, S cerevisiae DNA Repair Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sekelsky J J
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
McKim K S
Chin G M
Hawley R S
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/141.2.619
Published
1995-10-00
Pages
619-27
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206761
Subset
IM
Databases
GENBANK
U27181
External Links
PubMed source DOI: 10.1093/genetics/141.2.619 PMC Full Text View journal details
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