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PMID: 8655528 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of suppressor mutations of spoIVCA mutations: occurrence of DNA rearrangement in the absence of site-specific DNA recombinase SpoIVCA in Bacillus subtilis.

Journal of bacteriology ·Vol. 178 ·No. 11 ·1996-06-00 ·Pages 3380-3

Sato T, Harada K, Kobayashi Y

Abstract

The spoIVCA gene of Bacillus subtilis encodes a site-specific recombinase, which excises a 48-kb skin element from the chromosomal DNA by DNA rearrangement and creates a new composite gene, sigK, on the chromosome. From spoIVCA mutants, we have isolated Spo+ revertants which have no skin element but have an intact sigK gene. This result suggests that the DNA rearrangement can occur in the absence of spoIVCA.

MeSH Terms

Bacillus subtilis/genetics Base Sequence DNA Nucleotidyltransferases/genetics Gene Rearrangement Integrases Molecular Sequence Data Mutation Recombinases

Chemicals

Recombinases DNA Nucleotidyltransferases Integrases integron integrase IntI1

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sato T

Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.

Harada K

Kobayashi Y

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.178.11.3380-3383.1996

Published

1996-06-00

Pages

3380-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC178100

Subset

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1128/jb.178.11.3380-3383.1996 PMC Full Text View journal details

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Analysis of suppressor mutations of spoIVCA mutations: occurrence of DNA rearrangement in the absence of site-specific DNA recombinase SpoIVCA in Bacillus subtilis.

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