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PMID: 8666941 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The interaction of macrophage and non-macrophage tropic isolates of HIV-1 with thymic and tonsillar dendritic cells in vitro.

The Journal of experimental medicine ·Vol. 183 ·No. 4 ·1996-04-01 ·Pages 1851-6

Cameron PU, Lowe MG, Sotzik F, Coughlan AF, Crowe SM, Shortman K

Abstract

Dendritic cells isolated from thymus and tonsil were tested for susceptibility to HIV-1 strains that are tropic for macrophages or for T cell lines. DCs were purified by cell sorting and before infection expressed high levels of CD4 and HLA-DR and lacked markers for T, B, NK cells, or macrophages. Viral entry and reverse transcription was found after pulsing with strains of HIV-1 that could infect macrophages. During the first 36 h the PCR signals for gag sequences increased in DCs and macrophages. In contrast little if any viral DNA was found after pulsing macrophages or DCs with HIV-1 that was able to infect T cell lines. DCs pulsed with HIV-1 were able to transmit infection to responding T cells during an allogeneic or superantigen response. Selection for virus able to infect lymphoid DCs and other DCs expressing CD4 and its transfer to T cells during subsequent immune responses may provide a mechanism for the observed predominance of macrophage-tropic HIV-1 after in vivo transmission.

MeSH Terms
CD4 Antigens/analysis Cell Separation Child Dendritic Cells/virology Genes, gag HIV Reverse Transcriptase HIV-1/growth & development HLA-DR Antigens/analysis Humans Macrophages/virology Palatine Tonsil/cytology,virology RNA-Directed DNA Polymerase/metabolism T-Lymphocytes/virology Thymus Gland/cytology,virology Transcription, Genetic
Chemicals
CD4 Antigens HLA-DR Antigens HIV Reverse Transcriptase RNA-Directed DNA Polymerase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cameron P U
AIDS Pathogenesis Unit, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.
Lowe M G
Sotzik F
Coughlan A F
Crowe S M
Shortman K
References (27)
27 references, click to expand
  1. Genotypic and phenotypic characterization of HIV-1 patients with primary infection.
    Science. 1993 Aug 27;261(5125):1179-81 PMID: 8356453
  2. Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population.
    Nature. 1993 Apr 22;362(6422):761-3 PMID: 8469288
  3. B7/BB-1 is a leucocyte differentiation antigen on human dendritic cells induced by activation.
    Immunology. 1993 Aug;79(4):616-20 PMID: 8406586
  4. Assessment of CD4 expression by early T precursor cells and by dendritic cells in the human thymus.
    J Immunol. 1994 Apr 1;152(7):3370-7 PMID: 7511645
  5. Two subpopulations of human triple-negative thymic cells are susceptible to infection by human immunodeficiency virus type 1 in vitro.
    J Virol. 1994 May;68(5):3041-50 PMID: 7512158
  6. Infection and apoptotic cell death of CD4+ T cells during an immune response to HIV-1-pulsed dendritic cells.
    AIDS Res Hum Retroviruses. 1994 Jan;10(1):61-71 PMID: 7910031
  7. Susceptibility of dendritic cells to HIV-1 infection in vitro.
    J Leukoc Biol. 1994 Sep;56(3):257-65 PMID: 8083598
  8. CD4 and CD8 expression by human and mouse thymic dendritic cells.
    Immunol Lett. 1994 May;40(2):93-9 PMID: 8088877
  9. CD4-independent infection of human peripheral blood dendritic cells with isolates of human immunodeficiency virus type 1.
    J Gen Virol. 1993 Jul;74 ( Pt 7):1277-85 PMID: 8336119
  10. Dendritic cells freshly isolated from human blood express CD4 and mature into typical immunostimulatory dendritic cells after culture in monocyte-conditioned medium.
    J Exp Med. 1993 Sep 1;178(3):1067-76 PMID: 8102389
  11. Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1.
    Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):826-30 PMID: 7846060
  12. The role of CD40 and CD80 accessory cell molecules in dendritic cell-dependent HIV-1 infection.
    Immunity. 1994 Jul;1(4):317-25 PMID: 7534204
  13. Non-adherent, low-density cells from human peripheral blood contain dendritic cells and monocytes, both with veiled morphology.
    Immunology. 1986 Apr;57(4):595-603 PMID: 3007336
  14. Susceptibility of human peripheral blood dendritic cells to infection by human immunodeficiency virus.
    J Gen Virol. 1987 Apr;68 ( Pt 4):1177-81 PMID: 3646288
  15. Accessory cell requirements for the mixed-leukocyte reaction and polyclonal mitogens, as studied with a new technique for enriching blood dendritic cells.
    Cell Immunol. 1988 Jan;111(1):167-82 PMID: 2962741
  16. Decreased accessory cell function by human monocytic cells after infection with HIV.
    J Immunol. 1988 Mar 1;140(5):1485-9 PMID: 2964477
  17. Isolation and characterization of human tonsil dendritic cells.
    J Exp Med. 1988 Jul 1;168(1):157-70 PMID: 2456366
  18. Immunological abnormalities in human immunodeficiency virus (HIV)-infected asymptomatic homosexual men. HIV affects the immune system before CD4+ T helper cell depletion occurs.
    J Clin Invest. 1988 Dec;82(6):1908-14 PMID: 2974045
  19. Dendritic cell infection, depletion and dysfunction in HIV-infected individuals.
    Immunology. 1990 Sep;71(1):38-45 PMID: 2145214
  20. The distinct surface of human blood dendritic cells, as observed after an improved isolation method.
    Proc Natl Acad Sci U S A. 1990 Oct;87(19):7698-702 PMID: 2145580
  21. Multiple patterns of alloantigen presenting/stimulating cell dysfunction in patients with AIDS.
    J Immunol. 1991 Apr 1;146(7):2207-13 PMID: 1672347
  22. Morphology and phenotype of dendritic cells from peripheral blood and their productive and non-productive infection with human immunodeficiency virus type 1.
    Immunology. 1991 Mar;72(3):361-7 PMID: 1709140
  23. Replication of human immunodeficiency virus type 1 in primary dendritic cell cultures.
    Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7998-8002 PMID: 1910172
  24. The dendritic cell system and its role in immunogenicity.
    Annu Rev Immunol. 1991;9:271-96 PMID: 1910679
  25. Dendritic cells exposed to human immunodeficiency virus type-1 transmit a vigorous cytopathic infection to CD4+ T cells.
    Science. 1992 Jul 17;257(5068):383-7 PMID: 1352913
  26. Isolation and characterization of human peripheral blood dendritic cells.
    J Immunol. 1993 Feb 1;150(3):821-34 PMID: 7678623
  27. Human immunodeficiency virus infection of the human thymus and disruption of the thymic microenvironment in the SCID-hu mouse.
    J Exp Med. 1993 Oct 1;178(4):1151-63 PMID: 8376927
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.183.4.1851
Published
1996-04-01
Pages
1851-6
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192540
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.183.4.1851 PMC Full Text View journal details
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