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PMID: 8698039 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Engrailed and Hox homeodomain proteins contain a related Pbx interaction motif that recognizes a common structure present in Pbx.

The EMBO journal ·Vol. 15 ·No. 13 ·1996-07-01 ·Pages 3385-93

Peltenburg LT, Murre C

Abstract

Hox gene products have the ability to interact with either extradenticle or pbx gene products to bind cooperatively to DNA. The region in Hox proteins that is required for this interaction is located N-terminal of the homeodomain and contains a highly conserved hexapeptide. We now show that the engrailed gene products also contain a Pbx interaction motif positioned within a previously conserved region, the EH2 domain. The EH2 domain is located N-terminal of the homeodomain. Two tryptophan residues present in the Drosophila and murine Engrailed EH2 domain are required for cooperativity with extradenticle and Pbx, respectively. A second conserved domain, EH3, is required as well for cooperativity with Pbx, since deletions or an insertion in this region reduce cooperative DNA binding. Peptides containing the Pbx interaction motif of either Engrailed or Hox are capable of destabilizing Engrailed-Pbx and Hox-Pbx cooperative DNA binding. These data indicate that the Pbx interaction motifs present in Hox and engrailed gene products recognize a common structure present in the Pbx family of homeodomain proteins.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites Biological Evolution DNA Probes DNA-Binding Proteins/genetics,metabolism Drosophila Proteins Homeodomain Proteins/genetics,metabolism Humans Insect Hormones/genetics,metabolism Molecular Sequence Data Pre-B-Cell Leukemia Transcription Factor 1 Protein Binding Proto-Oncogene Proteins/metabolism Transcription Factors/genetics,metabolism
Chemicals
DNA Probes DNA-Binding Proteins Drosophila Proteins En protein, Drosophila Homeodomain Proteins Insect Hormones Pre-B-Cell Leukemia Transcription Factor 1 Proto-Oncogene Proteins Transcription Factors exd protein, Drosophila pbx1 protein, human
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peltenburg L T
Department of Biology 0366, University of California, San Diego, La Jolla, CA 92093, USA.
Murre C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-07-01
Pages
3385-93
Language
English
Region
England
NLM ID
8208664
PMCID
PMC451902
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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