Home LiteratureArticle Details
PMID: 8707826 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The BUD4 protein of yeast, required for axial budding, is localized to the mother/BUD neck in a cell cycle-dependent manner.

The Journal of cell biology ·Vol. 134 ·No. 2 ·1996-07-00 ·Pages 413-27

Sanders SL, Herskowitz I

Abstract

A and alpha cells of the yeast Saccharomyces cerevisiae exhibit an axial budding pattern, whereas a/alpha diploid cells exhibit a bipolar pattern. Mutations in BUD3, BUD4, and AXL1 cause a and alpha cells to exhibit the bipolar pattern, indicating that these genes are necessary to specify the axial budding pattern (Chant, J., and I. Herskowitz. 1991. Cell. 65:1203-1212; Fujita, A., C. Oka, Y. Arikawa, T. Katagi, A. Tonouchi, S. Kuhara, and Y. Misumi. 1994. Nature (Lond.). 372:567-570). We cloned and sequenced BUD4, which codes for a large, novel protein (Bud4p) with a potential GTP-binding motif. Bud4p is expressed and localized to the mother/bud neck in all cell types. Most mitotic cells contain two apparent rings of Bud4 immunoreactive staining, as observed for Bud3p (Chant, J., M. Mischke, E. Mitchell, I. Herskowitz, and J.R. Pringle. 1995. J. Cell Biol. 129: 767-778). Early G1 cells contain a single ring of Bud4p immunoreactive staining, whereas cells at START and in S phase lack these rings. The level of Bud4p is also regulated in a cell cycle-dependent manner. Bud4p is inefficiently localized in bud3 mutants and after a temperature shift of a temperature-sensitive mutant, cdc12, defective in the neck filaments. These observations suggest that Bud4p and Bud3p cooperate to recognize a spatial landmark (the neck filaments) during mitosis and support the hypothesis that they subsequently become a landmark for establishing the axial budding pattern in G1.

MeSH Terms
Amino Acid Sequence Animals Cell Cycle Cell Cycle Proteins/metabolism Cloning, Molecular Cytoskeletal Proteins Fungal Proteins/genetics,metabolism GTP-Binding Proteins/genetics,metabolism Humans Molecular Sequence Data Mutation Phenotype Rabbits Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
BUD3 protein, S cerevisiae BUD4 protein, S cerevisiae CDC12 protein, S cerevisiae Cell Cycle Proteins Cytoskeletal Proteins Fungal Proteins Saccharomyces cerevisiae Proteins GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sanders S L
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448, USA. [email protected]
Herskowitz I
References (73)
73 references, click to expand
  1. Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity.
    Mol Biol Cell. 1993 Dec;4(12):1307-16 PMID: 8167411
  2. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
    Anal Biochem. 1978 Jun 15;87(1):206-10 PMID: 98070
  3. Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3 gene product and the timing of events at the budding site.
    J Cell Biol. 1991 Feb;112(4):535-44 PMID: 1993729
  4. BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast.
    Nature. 1993 Sep 16;365(6443):269-74 PMID: 8371782
  5. Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation.
    J Bacteriol. 1975 Aug;123(2):693-703 PMID: 1097423
  6. Anillin, a contractile ring protein that cycles from the nucleus to the cell cortex.
    J Cell Biol. 1995 Oct;131(1):165-78 PMID: 7559773
  7. Patterns of bud-site selection in the yeast Saccharomyces cerevisiae.
    J Cell Biol. 1995 May;129(3):751-65 PMID: 7730409
  8. A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism.
    Mol Cell Biol. 1994 Jan;14(1):348-59 PMID: 8264602
  9. Courtship in S. cerevisiae: both cell types choose mating partners by responding to the strongest pheromone signal.
    Cell. 1990 Nov 30;63(5):1039-51 PMID: 2257622
  10. Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis.
    Exp Cell Res. 1971 Dec;69(2):265-76 PMID: 4950437
  11. Studies concerning the temporal and genetic control of cell polarity in Saccharomyces cerevisiae.
    J Cell Biol. 1991 Aug;114(3):515-32 PMID: 1860883
  12. Immunofluorescence methods for yeast.
    Methods Enzymol. 1991;194:565-602 PMID: 2005809
  13. The Drosophila peanut gene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins.
    Cell. 1994 May 6;77(3):371-9 PMID: 8181057
  14. S-phase feedback control in budding yeast independent of tyrosine phosphorylation of p34cdc28.
    Nature. 1992 Jan 23;355(6358):365-8 PMID: 1731250
  15. Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae.
    J Cell Biol. 1994 May;125(4):825-42 PMID: 8188749
  16. Members of the NAP/SET family of proteins interact specifically with B-type cyclins.
    J Cell Biol. 1995 Aug;130(3):661-73 PMID: 7622566
  17. Deduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of adenylate cyclase.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1251-5 PMID: 3081893
  18. A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription.
    Genes Dev. 1991 Dec;5(12B):2405-19 PMID: 1752436
  19. An essential G1 function for cyclin-like proteins in yeast.
    Cell. 1989 Dec 22;59(6):1127-33 PMID: 2574633
  20. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  21. A purified Drosophila septin complex forms filaments and exhibits GTPase activity.
    J Cell Biol. 1996 May;133(3):605-16 PMID: 8636235
  22. G1-specific cyclins of S. cerevisiae: cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinase.
    Cell. 1990 Jul 27;62(2):225-37 PMID: 2142620
  23. Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC11 gene product and the timing of events at the budding site.
    Dev Genet. 1991;12(4):281-92 PMID: 1934633
  24. Differential function and expression of Saccharomyces cerevisiae B-type cyclins in mitosis and meiosis.
    Mol Cell Biol. 1993 Apr;13(4):2113-25 PMID: 8455600
  25. Phenotypic analysis of temperature-sensitive yeast actin mutants.
    Cell. 1985 Feb;40(2):405-16 PMID: 3967297
  26. Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.
    Mol Biol Cell. 1992 Jul;3(7):805-18 PMID: 1387566
  27. Molecular analysis of a cell lineage.
    Nature. 1983 Apr 21;302(5910):670-6 PMID: 6339953
  28. Effect of reversible inhibition of deoxyribonucleic acid synthesis on the yeast cell cycle.
    J Bacteriol. 1973 Jan;113(1):263-70 PMID: 4120066
  29. Molecular cloning, characterization, and expression of human ADP-ribosylation factors: two guanine nucleotide-dependent activators of cholera toxin.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6101-5 PMID: 2474826
  30. A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis.
    Cell. 1991 Apr 5;65(1):163-74 PMID: 1849458
  31. Life cycle of the budding yeast Saccharomyces cerevisiae.
    Microbiol Rev. 1988 Dec;52(4):536-53 PMID: 3070323
  32. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  33. FtsZ ring structure associated with division in Escherichia coli.
    Nature. 1991 Nov 14;354(6349):161-4 PMID: 1944597
  34. Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9976-80 PMID: 2690082
  35. Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2.
    Genes Dev. 1992 Nov;6(11):2021-34 PMID: 1427070
  36. Cell polarization directed by extracellular cues in yeast.
    Mol Biol Cell. 1994 Nov;5(11):1169-75 PMID: 7865882
  37. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  38. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  39. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  40. Role of yeast insulin-degrading enzyme homologs in propheromone processing and bud site selection.
    Science. 1995 Oct 20;270(5235):464-7 PMID: 7569998
  41. Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients.
    J Cell Biol. 1995 Nov;131(4):845-61 PMID: 7490289
  42. Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS.
    Cell. 1992 Mar 20;68(6):1077-90 PMID: 1547504
  43. The GTPase superfamily: conserved structure and molecular mechanism.
    Nature. 1991 Jan 10;349(6305):117-27 PMID: 1898771
  44. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  45. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth.
    Genes Dev. 1994 Dec 15;8(24):2974-85 PMID: 8001818
  46. Interconversion of Yeast Mating Types III. Action of the Homothallism (HO) Gene in Cells Homozygous for the Mating Type Locus.
    Genetics. 1977 Mar;85(3):395-405 PMID: 17248736
  47. Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1.
    Cell. 1991 Jun 28;65(7):1213-24 PMID: 1905981
  48. The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence.
    J Biol Chem. 1981 Aug 10;256(15):8102-9 PMID: 7021545
  49. Control of the yeast cell cycle by the Cdc28 protein kinase.
    Curr Opin Cell Biol. 1993 Apr;5(2):166-79 PMID: 8507488
  50. Genetic control of the cell-division cycle in yeast. I. Detection of mutants.
    Proc Natl Acad Sci U S A. 1970 Jun;66(2):352-9 PMID: 5271168
  51. A yeast gene necessary for bud-site selection encodes a protein similar to insulin-degrading enzymes.
    Nature. 1994 Dec 8;372(6506):567-70 PMID: 7990931
  52. Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1987 Feb;84(3):779-83 PMID: 3543936
  53. Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway.
    Cell. 1991 Jun 28;65(7):1203-12 PMID: 2065354
  54. Cell cycle-regulated transcription of the CLB2 gene is dependent on Mcm1 and a ternary complex factor.
    Mol Cell Biol. 1995 Jun;15(6):3129-37 PMID: 7760809
  55. Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae.
    J Cell Biol. 1992 Aug;118(3):619-29 PMID: 1639847
  56. A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product.
    Nature. 1983 Dec 15-21;306(5944):704-7 PMID: 6318115
  57. Functions of microtubules in the Saccharomyces cerevisiae cell cycle.
    J Cell Biol. 1988 Oct;107(4):1409-26 PMID: 3049620
  58. FAR1 is required for oriented polarization of yeast cells in response to mating pheromones.
    J Cell Biol. 1995 Nov;131(4):863-73 PMID: 7490290
  59. A highly ordered ring of membrane-associated filaments in budding yeast.
    J Cell Biol. 1976 Jun;69(3):717-21 PMID: 773946
  60. Escherichia coli cell-division gene ftsZ encodes a novel GTP-binding protein.
    Nature. 1992 Sep 17;359(6392):251-4 PMID: 1528267
  61. The septins: roles in cytokinesis and other processes.
    Curr Opin Cell Biol. 1996 Feb;8(1):106-19 PMID: 8791410
  62. The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae.
    Cell. 1991 Apr 5;65(1):145-61 PMID: 1849457
  63. Escherichia coli cell division protein FtsZ is a guanine nucleotide binding protein.
    Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):1053-7 PMID: 8430073
  64. The essential bacterial cell-division protein FtsZ is a GTPase.
    Nature. 1992 Sep 17;359(6392):254-6 PMID: 1528268
  65. Specification of sites for polarized growth in Saccharomyces cerevisiae and the influence of external factors on site selection.
    Mol Biol Cell. 1992 Sep;3(9):1025-35 PMID: 1421575
  66. Symmetric cell division in pseudohyphae of the yeast Saccharomyces cerevisiae.
    Mol Biol Cell. 1994 Sep;5(9):1003-22 PMID: 7841518
  67. Role of Bud3p in producing the axial budding pattern of yeast.
    J Cell Biol. 1995 May;129(3):767-78 PMID: 7730410
  68. Development of cell polarity in budding yeast.
    Cell. 1991 Jun 28;65(7):1093-6 PMID: 1905977
  69. Guanine nucleotide-dependent assembly of FtsZ into filaments.
    J Bacteriol. 1994 May;176(9):2754-8 PMID: 8169229
  70. Staining of bud scars and other cell wall chitin with calcofluor.
    Methods Enzymol. 1991;194:732-5 PMID: 2005820
  71. Immunofluorescence localization of the Saccharomyces cerevisiae CDC12 gene product to the vicinity of the 10-nm filaments in the mother-bud neck.
    Mol Cell Biol. 1987 Oct;7(10):3678-87 PMID: 3316985
  72. Spindle orientation and asymmetric cell fate.
    Cell. 1995 Aug 25;82(4):523-6 PMID: 7664329
  73. Components required for cytokinesis are important for bud site selection in yeast.
    J Cell Biol. 1993 Jul;122(2):373-86 PMID: 8320260
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.134.2.413
Email
[email protected]
Published
1996-07-00
Pages
413-27
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120863
Subset
IM
Grants
NIGMS NIH HHS · GM48052 · United States
Databases
GENBANK
U41641
External Links
PubMed source DOI: 10.1083/jcb.134.2.413 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2