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PMID: 8709209 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Various modes of basic helix-loop-helix protein-mediated regulation of murine leukemia virus transcription in lymphoid cell lines.

Journal of virology ·Vol. 70 ·No. 9 ·1996-09-00 ·Pages 5893-901

Nielsen AL, Nørby PL, Pedersen FS, Jørgensen P

Abstract

The transcriptionally regulatory regions of the lymphomagenic Akv and SL3-3 murine leukemia retroviruses (MLVs) contain two types of E-box consensus motifs, CAGATG. One type, EA/S, is located in the upstream promoter region, and the other, E(gre), is located in a tandem repeat with enhancer properties. We have examined the requirements of the individual E-boxes in MLV transcriptional regulation. In lymphoid cell lines only, the E(gre)-binding protein complexes included ALF1 or HEB and E2A basic helix-loop-helix proteins. Ectopic ALF1 and E2A proteins required intact E(gre) motifs for mediating transcriptional activation. ALF1 transactivated transcription of Akv MLV through the two E(gre) motifs equally, whereas E2A protein required the promoter-proximal E(gre) motif. In T- and B-cell lines, the E(gre) motifs were of major importance for Akv MLV transcriptional activity, while the EA/S motif had some effect. In contrast, neither E(gre) nor EA/S motifs contributed pronouncedly to Akv MLV transcription in NIH 3T3 cells lacking DNA-binding ALF1 or HEB and E2A proteins. The Id1 protein was found to repress ALF1 activity in vitro and in vivo. Moreover, ectopic Id1 repressed E(gre)-directed but not EA/S-directed MLV transcription in lymphoid cell lines. In conclusion, E(gre) motifs and interacting basic helix-loop-helix proteins are important determinants for MLV transcriptional activity in lymphocytic cell lines.

MeSH Terms
3T3 Cells Adenovirus E2 Proteins/biosynthesis Animals B-Lymphocytes Base Sequence Basic Helix-Loop-Helix Transcription Factors Cell Line Chloramphenicol O-Acetyltransferase/biosynthesis Consensus Sequence DNA Primers DNA-Binding Proteins/biosynthesis Enhancer Elements, Genetic HeLa Cells Helix-Loop-Helix Motifs Humans Leukemia Virus, Murine/genetics,metabolism Lymphoma/virology Mice Molecular Sequence Data Mutagenesis, Site-Directed Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Transcription Factors/biosynthesis Transcription, Genetic Transfection
Chemicals
Adenovirus E2 Proteins Basic Helix-Loop-Helix Transcription Factors DNA Primers DNA-Binding Proteins Recombinant Fusion Proteins Tcf12 protein, mouse Transcription Factors TCF12 protein, human Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nielsen A L
Department of Molecular and Structural Biology, University of Aarhus, Denmark.
Nørby P L
Pedersen F S
Jørgensen P
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44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.9.5893-5901.1996
Published
1996-09-00
Pages
5893-901
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190607
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.9.5893-5901.1996 PMC Full Text View journal details
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