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PMID: 8758895 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of an H2-M3-restricted Listeria epitope: implications for antigen presentation by M3.

Immunity ·Vol. 5 ·No. 1 ·1996-07-00 ·Pages 63-72

Lenz LL, Dere B, Bevan MJ

Abstract

Using expression cloning, we have identified an H2-M3-restricted epitope of the intracellular bacterial pathogen Listeria monocytogenes. Picomolar concentrations of an amino-terminal N-formylated hexapeptide, fMIGWII, targeted cells for lysis by CD8+ cytotoxic T cells, while the nonformylated peptide was approximately 100-fold less active. The sequence of the 185 aa protein source of this epitope predicts a transmembrane protein that retains its N terminus and assumes an N(out)-C(in) topology. This membrane orientation offers an explanation for the protection of the epitope from deformylases present in the bacterial cell and suggests an explanation for the ability of phagocytes to present H2-M3-restricted bacterial epitopes via a vacuolar TAP-independent mechanism.

MeSH Terms
Alleles Amino Acid Sequence Animals Antigen Presentation/genetics Antigens, Bacterial/genetics,immunology,isolation & purification Bacterial Proteins/genetics,immunology,isolation & purification Base Sequence Cytotoxicity, Immunologic/genetics Epitopes/genetics,immunology,isolation & purification Gene Library H-2 Antigens/genetics,immunology Listeria monocytogenes/genetics,immunology Membrane Proteins/genetics,immunology,isolation & purification Mice Mice, Inbred C57BL Molecular Sequence Data T-Lymphocytes, Cytotoxic/immunology Transformation, Bacterial/immunology
Chemicals
Antigens, Bacterial Bacterial Proteins Epitopes H-2 Antigens LemA protein, Listeria Membrane Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lenz L L
Department of Immunology, University of Washington, Seattle, 98195, USA.
Dere B
Bevan M J
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Article Info
Journal
Immunity
Abbr.
Immunity
ISSN
1074-7613
DOI
10.1016/s1074-7613(00)80310-6
Published
1996-07-00
Pages
63-72
Language
English
Region
United States
NLM ID
9432918
PMCID
PMC2778046
Subset
IM
Grants
NCI NIH HHS · CA-09537 · United States
NIAID NIH HHS · R01 AI019335 · United States
NCI NIH HHS · T32 CA009537-13 · United States
NIAID NIH HHS · R01 AI019335-19 · United States
Howard Hughes Medical Institute · United States
NCI NIH HHS · T32 CA009537 · United States
NIAID NIH HHS · AI-19335 · United States
Databases
GENBANK
U66186
External Links
PubMed source DOI: 10.1016/s1074-7613(00)80310-6 PMC Full Text View journal details
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