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PMID: 8769408 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The periphery of nuclear domain 10 (ND10) as site of DNA virus deposition.

The Journal of cell biology ·Vol. 134 ·No. 4 ·1996-08-00 ·Pages 815-26

Ishov AM, Maul GG

Abstract

After DNA viruses enter the nucleus, they initiate a transcriptional cascade which is followed by replication. We investigated whether these processes take place at specific nuclear sites or, as suggested by the mode of entry, randomly throughout the nucleus. Three distinct nuclear domains, nuclear factor-1 sites, coiled bodies, and nuclear domain 10 (ND10), were used as markers to investigate the relative position of DNA virus replication sites. We found that all three nuclear domains had a very high spatial correlation with each other in uninfected cells. After adenoviral infection, nuclear factor 1 and coiled bodies were found associated with some viral replication domains. Simian virus 40 begins replication adjacent to ND10 but adenovirus 5 and herpes simplex type 1 modified ND10s before replication. Adenovirus E4orf 3 gene deletion mutants retain ND10 and begin replication at the peripheries of ND10. The same was found for the herpes simplex virus type 1 immediate early gene 1 mutants. That the deposition and replication of adenovirus 5 and herpesvirus type 1 at ND10 was not a mutant phenotype was confirmed by finding the input wild-type virus juxtaposed to ND10. The transport of viral genomes to ND10 does not require viral gene expression. Thus, the peripheries of ND10 represent preferred sites where early steps of transcription and replication of at least three DNA virus families take place, suggesting a new set of functional properties for this large nuclear domain.

MeSH Terms
Binding Sites CCAAT-Enhancer-Binding Proteins Cell Nucleus/virology DNA Replication/physiology DNA Viruses/physiology DNA, Viral/analysis DNA-Binding Proteins/analysis Humans NFI Transcription Factors Nuclear Proteins/analysis RNA, Viral/analysis Transcription Factors/analysis Tumor Cells, Cultured Viral Proteins/analysis Virus Replication/physiology Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA, Viral DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins RNA, Viral Transcription Factors Viral Proteins Y-Box-Binding Protein 1 YBX1 protein, human
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ishov A M
Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
Maul G G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.134.4.815
Published
1996-08-00
Pages
815-26
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120958
Subset
IM
Grants
NCI NIH HHS · CA-10815 · United States
External Links
PubMed source DOI: 10.1083/jcb.134.4.815 PMC Full Text View journal details
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