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PMID: 8770187 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Visualization of beta-sheets and side-chain clusters in two-dimensional periodic arrays of streptavidin on phospholipid monolayers by electron crystallography.

Biophysical journal ·Vol. 70 ·No. 1 ·1996-01-00 ·Pages 57-68

Avila-Sakar AJ, Chiu W

Abstract

The biotin-binding protein streptavidin was crystallized as two-dimensional periodic arrays on biotinylated phospholipid monolayers. Electron diffraction patterns and images of the arrays embedded in vitreous ice were recorded to near-atomic resolution. Amplitudes and phases of structure factors were computed and combined to produce a 3 A projection density map. The reliability of the map was verified by comparing it to the available x-ray atomic model of the molecule. Projection densities from beta-strands and some amino acid side chains were identified from the electron cryomicroscopy map. These results demonstrate the first near-atomic image of this type of protein periodic array by electron crystallography, which has a great potential to aid in the structural characterization of molecular arrays engineered on a monolayer for various basic or biotechnological applications.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry Biophysical Phenomena Biophysics Crystallography/methods Crystallography, X-Ray Electrons Models, Molecular Molecular Sequence Data Molecular Structure Phospholipids Protein Conformation Protein Structure, Secondary Streptavidin
Chemicals
Bacterial Proteins Phospholipids Streptavidin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Avila-Sakar A J
Department of Molecular Physiology and Biophysics, W. M. Keck Center for Computational Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Chiu W
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(96)79597-8
Published
1996-01-00
Pages
57-68
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1224909
Subset
IM
Grants
NCRR NIH HHS · RR02250 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1016/S0006-3495(96)79597-8 PMC Full Text View journal details
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