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PMID: 8789021 Published · ppublish English Comparative Study Journal Article

Typing of Mycobacterium avium isolates by PCR.

Journal of clinical microbiology ·Vol. 34 ·No. 2 ·1996-02-00 ·Pages 389-92

Picardeau M, Vincent V

Abstract

A Mycobacterium avium typing method based on PCR amplification of genomic sequences located between the recently described repetitive elements IS1245 and IS1311 was developed. This method was applied to a set of epidemiologically related and unrelated strains and compared with restriction fragment length polymorphism analysis with IS1245 as the probe. This PCR typing consists of a rapid and simple technique, providing a reproducible M. avium characterization as discriminant as restriction fragment length polymorphism analysis.

MeSH Terms
AIDS-Related Opportunistic Infections/complications,microbiology Bacterial Typing Techniques Base Sequence DNA Primers/genetics DNA, Bacterial/genetics Humans Molecular Sequence Data Mycobacterium avium Complex/classification,genetics,isolation & purification Mycobacterium avium-intracellulare Infection/complications,microbiology Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid
Chemicals
DNA Primers DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Picardeau M
Laboratoire de Référence des Mycobactéries, Institut Pasteur, Paris, France.
Vincent V
References (14)
14 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1996-02-00
Pages
389-92
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC228803
Subset
IM
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