Abstract
A Mycobacterium avium typing method based on PCR amplification of genomic sequences located between the recently described repetitive elements IS1245 and IS1311 was developed. This method was applied to a set of epidemiologically related and unrelated strains and compared with restriction fragment length polymorphism analysis with IS1245 as the probe. This PCR typing consists of a rapid and simple technique, providing a reproducible M. avium characterization as discriminant as restriction fragment length polymorphism analysis.
MeSH Terms
AIDS-Related Opportunistic Infections/complications,microbiology
Bacterial Typing Techniques
Base Sequence
DNA Primers/genetics
DNA, Bacterial/genetics
Humans
Molecular Sequence Data
Mycobacterium avium Complex/classification,genetics,isolation & purification
Mycobacterium avium-intracellulare Infection/complications,microbiology
Polymerase Chain Reaction/methods
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid
Chemicals
DNA Primers
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Picardeau M
Laboratoire de Référence des Mycobactéries, Institut Pasteur, Paris, France.
Vincent V
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