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PMID: 8794333 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A novel simian virus 40 early-region domain mediates transactivation of the cyclin A promoter by small-t antigen and is required for transformation in small-t antigen-dependent assays.

Journal of virology ·Vol. 70 ·No. 10 ·1996-10-00 ·Pages 6902-8

Porrás A, Bennett J, Howe A, Tokos K, Bouck N, Henglein B, Sathyamangalam S, Thimmapaya B, Rundell K

Abstract

At least three regions of the simian virus 40 small-t antigen (small-t) contribute to the protein's ability to enhance cellular transformation. As we showed previously for rat F111 cells, one region includes sequences from residues 97 to 103 that are involved in the binding and inhibition of protein phosphatase 2A. In the present study, the role of the protein phosphatase 2A binding region was confirmed in two additional small-t-dependent transformation systems. Second, small-t was found to provide a function previously identified as a large-T transformation domain. Mutations in residues 19 to 28 of large-T affected its transforming ability, but these mutations were complemented by a wild-type small-t. A third region of small-t was also required for efficient transformation. This region, the 42-47 region, is shared by large-T and small-t and contains a conserved HPDKGG hexapeptide. The 42-47 region function could be provided by either small-t or large-T in small-t-dependent systems. Mutations in the 42-47 region reduced the ability of small-t to transactivate the cyclin A promoter, of interest because small-t increased endogenous cyclin A mRNA levels in both human and monkey cells, as well as transactivating the promoter in transient assays.

MeSH Terms
Animals Antigens, Viral, Tumor/genetics Cell Line Cell Transformation, Viral Cyclins/genetics Humans Mutation Promoter Regions, Genetic/genetics Rats Simian virus 40/genetics,metabolism Transcriptional Activation
Chemicals
Antigens, Viral, Tumor Cyclins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Porrás A
Department of Microbiology-Immunology, Northwestern University Medical Center, Chicago, Illinois 60611, USA.
Bennett J
Howe A
Tokos K
Bouck N
Henglein B
Sathyamangalam S
Thimmapaya B
Rundell K
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44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.10.6902-6908.1996
Published
1996-10-00
Pages
6902-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190739
Subset
IM
Grants
NIAID NIH HHS · AI-20156 · United States
NCI NIH HHS · CA-21327 · United States
NCI NIH HHS · CA-52750 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.10.6902-6908.1996 PMC Full Text View journal details
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