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PMID: 880330 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The organization of repeated nucleotide sequences in the replicons of mammalian DNA.

Biophysical journal ·Vol. 19 ·No. 2 ·1977-08-00 ·Pages 117-23

Mattern MR, Painter RB

Abstract

Chinese hamster ovary cells were irradiated with 100-5,000 rads of X-rays and inhibition of the initiation of replicons after irradiation was demonstrated by analyzing nascent DNA sedimented in alkaline sucrose gradients. The renaturation kinetics of DNA synthesized during 60 min of incubation after irradiation was compared with that of DNA synthesized during the 60 min after sham irradiation and with that of parental DNA. Nascent DNA from cells whose replicon initiation was inhibited renatured faster than nascent DNA from control cells in the COt range of repeated nucleotide sequences, suggesting that regions of the replicon not close to origins are enriched in repeated sequences and that regions close to origins are enriched in unique sequences. A class of repeated nucleotide sequences may be involved in the regulation of replicon initiation.

MeSH Terms
Base Sequence Cell Line DNA/isolation & purification,metabolism DNA Replication/radiation effects Deoxyribonucleotides/analysis Kinetics Nucleic Acid Renaturation X-Rays
Chemicals
Deoxyribonucleotides DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mattern M R
Painter R B
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24 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(77)85574-4
Published
1977-08-00
Pages
117-23
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1473308
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(77)85574-4 PMC Full Text View journal details
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