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PMID: 880841 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes.

Chromosoma ·Vol. 61 ·No. 4 ·1977-06-23 ·Pages 335-44

Yunis JJ, Kuo MT, Saunders GF

Abstract

Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.

MeSH Terms
Cell Line Chromosome Mapping DNA Humans Lymphocytes Nucleic Acid Hybridization RNA, Messenger Spleen Transcription, Genetic
Chemicals
RNA, Messenger DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yunis J J
Kuo M T
Saunders G F
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23 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00288617
Published
1977-06-23
Pages
335-44
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00288617 View journal details
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