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PMID: 8832904 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proteins binding to the promoter region of the operon encoding the major outer surface proteins OspA and OspB of Borrelia burgdorferi.

Molecular biology reports ·Vol. 21 ·No. 3 ·1995-00-00 ·Pages 159-64

Margolis N, Samuels DS

Abstract

The synthesis of the major outer surface proteins OspA and OspB in Borrelia burgdorferi varies among strains and during in vitro cultivation. We examined B. burgdorferi CA-11.2A for the presence of proteins that bind to the ospAB operon promoter region. Three major DNA-protein complexes were detected using a mobility shift assay; one of these complexes was due to sequence-specific binding. These proteins may be involved in the regulation of ospAB transcription and the pathogenesis of Lyme disease.

MeSH Terms
Antigens, Bacterial Antigens, Surface/genetics Bacterial Outer Membrane Proteins/genetics Bacterial Vaccines Binding, Competitive Borrelia burgdorferi Group/genetics DNA, Bacterial/metabolism DNA-Binding Proteins/metabolism Lipoproteins Operon/genetics Promoter Regions, Genetic/genetics
Chemicals
Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Bacterial Vaccines DNA, Bacterial DNA-Binding Proteins Lipoproteins OspA protein OspB protein, Borrelia burgdorferi
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Margolis N
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA.
Samuels D S
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00997238
Published
1995-00-00
Pages
159-64
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00997238 View journal details
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