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PMID: 8879213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of dendritic cell numbers and maturation by lipopolysaccharide in vivo.

The Journal of experimental medicine ·Vol. 184 ·No. 4 ·1996-10-01 ·Pages 1413-24

De Smedt T, Pajak B, Muraille E, Lespagnard L, Heinen E, De Baetselier P, Urbain J, Leo O, Moser M

Abstract

Dendritic cells (DC) are described as "nature's adjuvant," since they have the capacity to sensitize T cells in vivo upon first encounter with the antigen. The potent accessory properties of DC appear to develop sequentially. In particular, the ability to process antigens and to sensitize native T cells develops in sequence, a process termed "maturation" that is well described in vitro. Here, we obtain evidence for maturation in vivo in response to the bacterial product lipopolysaccharide (LPS). Before LPS treatment, many DC are found at the margin between the red and white pulp. These cells lack the M342 and DEC-205 markers, but process soluble proteins effectively. 6 h after LPS, DC with the M342 and DEC-205 markers are found in increased numbers in the T cell areas. These cells have a reduced capacity to process proteins, but show increases in the B7 costimulator and T cell stimulatory capacity. 48 h after LPS, the number of DC in the spleen is reduced markedly. We interpret these findings to mean that LPS can cause DC in the marginal zone to mature and to migrate into and then out of the T cell areas.

MeSH Terms
Animals Antigens, Differentiation B7-1 Antigen/biosynthesis Cell Differentiation Cell Movement Cell Separation Dendritic Cells/drug effects,immunology Female Flow Cytometry Histocompatibility Antigens Class II/biosynthesis Immunohistochemistry Lipopolysaccharides/pharmacology Lymphocyte Activation Mice Mice, Inbred BALB C Mice, Inbred CBA Spleen/anatomy & histology,drug effects,immunology Up-Regulation
Chemicals
Antigens, Differentiation B7-1 Antigen Histocompatibility Antigens Class II Lipopolysaccharides
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
De Smedt T
Département de Biologie Moléculaire, Université Libre de Bruxelles, Belgium.
Pajak B
Muraille E
Lespagnard L
Heinen E
De Baetselier P
Urbain J
Leo O
Moser M
References (65)
65 references, click to expand
  1. Immunosuppression in vivo by a soluble form of the CTLA-4 T cell activation molecule.
    Science. 1992 Aug 7;257(5071):792-5 PMID: 1496399
  2. The role of prostaglandin E2 in immune suppression following injury.
    Ann Surg. 1991 Sep;214(3):253-62; discussion 262-3 PMID: 1929607
  3. Clustering of dendritic cells, helper T lymphocytes, and histocompatible B cells during primary antibody responses in vitro.
    J Exp Med. 1984 Sep 1;160(3):858-76 PMID: 6206192
  4. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones.
    Nature. 1992 Apr 16;356(6370):607-9 PMID: 1313950
  5. Migration of dendritic leukocytes from cardiac allografts into host spleens. A novel pathway for initiation of rejection.
    J Exp Med. 1990 Jan 1;171(1):307-14 PMID: 2404081
  6. Restoration of immunogenicity to passenger cell-depleted kidney allografts by the addition of donor strain dendritic cells.
    J Exp Med. 1982 Jan 1;155(1):31-41 PMID: 7033437
  7. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes.
    Cell Immunol. 1995 Jun;163(1):148-56 PMID: 7758125
  8. Phenotypic and functional characteristics of in vivo-activated Langerhans cells.
    J Immunol. 1990 Nov 1;145(9):2791-6 PMID: 2170524
  9. Studies on the density, distribution, and surface phenotype of intraepithelial class II major histocompatibility complex antigen (Ia)-bearing dendritic cells (DC) in the conducting airways.
    J Exp Med. 1991 Jun 1;173(6):1345-56 PMID: 2033368
  10. Tumour necrosis factor-alpha is required for accumulation of dendritic cells in draining lymph nodes and for optimal contact sensitization.
    Immunology. 1995 Jan;84(1):31-5 PMID: 7890303
  11. Primary stimulation by dendritic cells induces antiviral proliferative and cytotoxic T cell responses in vitro.
    J Exp Med. 1989 Apr 1;169(4):1255-64 PMID: 2784483
  12. Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ.
    J Exp Med. 1990 Aug 1;172(2):631-40 PMID: 2373994
  13. Evidence for an additional ligand, distinct from B7, for the CTLA-4 receptor.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11182-6 PMID: 7504299
  14. Specialized antigen-presenting cells. Splenic dendritic cells and peritoneal-exudate cells induced by mycobacteria activate effector T cells that are resistant to suppression.
    J Exp Med. 1982 May 1;155(5):1344-56 PMID: 6461712
  15. The role of dendritic cells in the initiation of immune responses to contact sensitizers. II. Studies in nude mice.
    Cell Immunol. 1985 Sep;94(2):435-9 PMID: 3875419
  16. Migration patterns of dendritic cells in the mouse. Homing to T cell-dependent areas of spleen, and binding within marginal zone.
    J Exp Med. 1988 Feb 1;167(2):646-51 PMID: 3258010
  17. Small amounts of superantigen, when presented on dendritic cells, are sufficient to initiate T cell responses.
    J Exp Med. 1993 Aug 1;178(2):633-42 PMID: 8340760
  18. Long-term survival of xenogeneic pancreatic islet grafts induced by CTLA4lg.
    Science. 1992 Aug 7;257(5071):789-92 PMID: 1323143
  19. The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus.
    Cell Immunol. 1989 Jan;118(1):108-25 PMID: 2910499
  20. Abolition of specific immune response defect by immunization with dendritic cells.
    Nature. 1985 Nov 7-13;318(6041):59-62 PMID: 2932649
  21. Identification of an alternative CTLA-4 ligand costimulatory for T cell activation.
    Science. 1993 Nov 5;262(5135):905-7 PMID: 7694361
  22. Expression of cytokine mRNA in murine splenic dendritic cells and better induction of T cell-derived cytokines by dendritic cells than by macrophages during in vitro costimulation assay using specific antigens.
    J Leukoc Biol. 1995 Feb;57(2):310-6 PMID: 7531747
  23. Endotoxin-mediated dendritic cell release from the intestine. Characterization of released dendritic cells and TNF dependence.
    J Immunol. 1995 Feb 1;154(3):1317-22 PMID: 7822800
  24. Immunogenicity signals 1,2,3 ... and 0.
    Immunol Today. 1989 Sep;10(9):283-6 PMID: 2590379
  25. A cell culture model for T lymphocyte clonal anergy.
    Science. 1990 Jun 15;248(4961):1349-56 PMID: 2113314
  26. Identification of monoclonal antibodies specific for the T cell receptor complex by Fc receptor-mediated CTL lysis.
    J Immunol. 1986 Dec 15;137(12):3874-80 PMID: 3097135
  27. Dendritic cell loss from nonlymphoid tissues after systemic administration of lipopolysaccharide, tumor necrosis factor, and interleukin 1.
    J Exp Med. 1995 Jun 1;181(6):2237-47 PMID: 7760009
  28. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody.
    J Exp Med. 1986 Apr 1;163(4):981-97 PMID: 3950549
  29. Distinct roles for the costimulatory ligands B7-1 and B7-2 in T helper cell differentiation?
    Cell. 1995 Jun 30;81(7):979-82 PMID: 7541314
  30. Processing and presentation of insulin. I. Analysis of immunogenic peptides and processing requirements for insulin A loop-specific T cells.
    J Immunol. 1987 Dec 15;139(12):3955-63 PMID: 2447156
  31. Antigen-pulsed dendritic cells can efficiently induce an antibody response in vivo.
    J Exp Med. 1992 Jan 1;175(1):15-21 PMID: 1370527
  32. Microbial induction of co-stimulatory activity for CD4 T-cell growth.
    Int Immunol. 1991 Apr;3(4):323-32 PMID: 1831651
  33. Long-term acceptance of major histocompatibility complex mismatched cardiac allografts induced by CTLA4Ig plus donor-specific transfusion.
    J Exp Med. 1993 Nov 1;178(5):1801-6 PMID: 8228826
  34. The differential effects of bacterial lipopolysaccharide (LPS) on splenic non-lymphoid cells demonstrated by monoclonal antibodies.
    Immunology. 1986 Jun;58(2):285-90 PMID: 3519443
  35. Granulocyte/macrophage colony-stimulating factor and interleukin 1 mediate the maturation of murine epidermal Langerhans cells into potent immunostimulatory dendritic cells.
    J Exp Med. 1988 Feb 1;167(2):700-5 PMID: 3279156
  36. Dermal tumour necrosis factor-alpha induces dendritic cell migration to draining lymph nodes, and possibly provides one stimulus for Langerhans' cell migration.
    Immunology. 1992 Feb;75(2):257-63 PMID: 1551688
  37. Two populations of splenic dendritic cells detected with M342, a new monoclonal to an intracellular antigen of interdigitating dendritic cells and some B lymphocytes.
    J Leukoc Biol. 1992 Jul;52(1):34-42 PMID: 1379295
  38. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.
    J Exp Med. 1990 May 1;171(5):1753-71 PMID: 2185332
  39. The Fas death factor.
    Science. 1995 Mar 10;267(5203):1449-56 PMID: 7533326
  40. Identification of a monoclonal antibody specific for a murine T3 polypeptide.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8 PMID: 2950524
  41. Migration patterns of dendritic cells in the mouse. Traffic from the blood, and T cell-dependent and -independent entry to lymphoid tissues.
    J Exp Med. 1988 Feb 1;167(2):632-45 PMID: 3258009
  42. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen.
    J Immunol Methods. 1990 Oct 4;133(1):55-66 PMID: 2145370
  43. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice.
    J Exp Med. 1983 Feb 1;157(2):613-27 PMID: 6185614
  44. T-cell activation by the CD28 ligand B7 is required for cardiac allograft rejection in vivo.
    Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):11102-5 PMID: 1332070
  45. Two tumour necrosis factor receptors: structure and function.
    Trends Cell Biol. 1995 Oct;5(10):392-9 PMID: 14732063
  46. Localization of antigen on lymph node dendritic cells after exposure to the contact sensitizer fluorescein isothiocyanate. Functional and morphological studies.
    J Exp Med. 1987 Dec 1;166(6):1654-67 PMID: 3119761
  47. Murine dendritic cells pulsed in vitro with tumor antigen induce tumor resistance in vivo.
    Eur J Immunol. 1994 Mar;24(3):605-10 PMID: 8125131
  48. Direct activation of CD8+ cytotoxic T lymphocytes by dendritic cells.
    J Exp Med. 1987 Jul 1;166(1):182-94 PMID: 2955069
  49. Presentation of antigen by mixed isotype class II molecules in normal H-2d mice.
    J Exp Med. 1992 Jan 1;175(1):157-62 PMID: 1730914
  50. The dendritic cell system and its role in immunogenicity.
    Annu Rev Immunol. 1991;9:271-96 PMID: 1910679
  51. Functional expression of the costimulatory molecule, B7/BB1, on murine dendritic cell populations.
    J Exp Med. 1992 Oct 1;176(4):1215-20 PMID: 1328465
  52. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.
    J Exp Med. 1994 Nov 1;180(5):1849-60 PMID: 7525841
  53. Prevention of rejection of murine islet allografts by pretreatment with anti-dendritic cell antibody.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3864-8 PMID: 6427778
  54. Interleukin 1 enhances T-dependent immune responses by amplifying the function of dendritic cells.
    J Exp Med. 1987 Feb 1;165(2):515-30 PMID: 2950198
  55. The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing.
    Nature. 1995 May 11;375(6527):151-5 PMID: 7753172
  56. Presentation of exogenous protein antigens by dendritic cells to T cell clones. Intact protein is presented best by immature, epidermal Langerhans cells.
    J Exp Med. 1989 Mar 1;169(3):1169-78 PMID: 2522497
  57. Expression and functional significance of an additional ligand for CTLA-4.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11054-8 PMID: 7504292
  58. Glucocorticoids down-regulate dendritic cell function in vitro and in vivo.
    Eur J Immunol. 1995 Oct;25(10):2818-24 PMID: 7589077
  59. Antigen processing in populations of mature murine dendritic cells is caused by subsets of incompletely matured cells.
    J Immunol. 1995 Jul 1;155(1):93-100 PMID: 7602127
  60. Failure or success in the restoration of virus-specific cytotoxic T lymphocyte response defects by dendritic cells.
    J Immunol. 1988 May 1;140(9):3186-93 PMID: 2834454
  61. CTLA-4 is a second receptor for the B cell activation antigen B7.
    J Exp Med. 1991 Sep 1;174(3):561-9 PMID: 1714933
  62. Polymicrobial sepsis but not low-dose endotoxin infusion causes decreased splenocyte IL-2/IFN-gamma release while increasing IL-4/IL-10 production.
    J Surg Res. 1994 Jun;56(6):579-85 PMID: 8015314
  63. Modulation of cytokine expression in mouse dendritic cell clones.
    Eur J Immunol. 1994 Oct;24(10):2522-6 PMID: 7925580
  64. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products.
    J Exp Med. 1995 Aug 1;182(2):389-400 PMID: 7629501
  65. The effects of hemorrhage and trauma on interleukin 2 production.
    Arch Surg. 1985 Dec;120(12):1341-4 PMID: 3877497
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.184.4.1413
Published
1996-10-01
Pages
1413-24
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192842
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.184.4.1413 PMC Full Text View journal details
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