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PMID: 8885235 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CDC37 is required for p60v-src activity in yeast.

Molecular biology of the cell ·Vol. 7 ·No. 9 ·1996-09-00 ·Pages 1405-17

Dey B, Lightbody JJ, Boschelli F

Abstract

Mutations in genes encoding the molecular chaperones Hsp90 and Ydj1p suppress the toxicity of the protein tyrosine kinase p60v-src in yeast by reducing its levels or its kinase activity. We describe isolation and characterization of novel p60v-src-resistant, temperature-sensitive cdc37 mutants, cdc37-34 and cdc37-17, which produce less p60v-src than the parental wild-type strain at 23 degrees C. However, p60v-src levels are not low enough to account for the resistance of these strains. Asynchronously growing cdc37-34 and cdc37-17 mutants arrest in G1 and G2/M when shifted from permissive temperatures (23 degrees C) to the restrictive temperature (37 degrees C), but hydroxyurea-synchronized cdc37-34 and cdc37-17 mutants arrest in G2/M when released from the hydroxyurea block and shifted from 23 to 37 degrees C. The previously described temperature-sensitive cdc37-1 mutant is p60v-src-sensitive and produces wild-type amounts of p60v-src at permissive temperatures but becomes p60v-src-resistant at its restrictive temperature, 38 degrees C. In all three cdc37 mutants, inactivation of Cdc37p by incubation at 38 degrees C reduces p60v-src-dependent tyrosine phosphorylation of yeast proteins to low or undetectable levels. Also, p60v-src levels are enriched in urea-solubilized extracts and depleted in detergent-solubilized extracts of all three cdc37 mutants prepared from cells incubated at the restrictive temperature. These results suggest that Cdc37p is required for maintenance of p60v-src in a soluble, biologically active form.

MeSH Terms
Cell Cycle Proteins/genetics,metabolism Drosophila Proteins Enzyme Activation G1 Phase/genetics G2 Phase/genetics Gene Expression Regulation, Fungal Genes, Lethal Genes, Suppressor Mitosis/genetics Molecular Chaperones Mutation Oncogene Protein pp60(v-src)/chemistry,genetics,metabolism Phosphorylation Sodium Dodecyl Sulfate/chemistry Solubility Temperature Tyrosine/metabolism Urea/chemistry Yeasts/genetics
Chemicals
Cell Cycle Proteins Drosophila Proteins Molecular Chaperones Sodium Dodecyl Sulfate Tyrosine Urea Oncogene Protein pp60(v-src)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dey B
Department of Biochemistry, Wayne State University Medical School, Detroit, Michigan 48201, USA.
Lightbody J J
Boschelli F
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.7.9.1405
Published
1996-09-00
Pages
1405-17
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275990
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.7.9.1405 PMC Full Text View journal details
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