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PMID: 2439705 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

pp60c-src has less affinity for the detergent-insoluble cellular matrix than do pp60v-src and other viral protein-tyrosine kinases.

Journal of virology ·Vol. 61 ·No. 8 ·1987-08-00 ·Pages 2420-7

Loeb DM, Woolford J, Beemon K

Abstract

A difference in affinity for a Nonidet P-40-insoluble cellular matrix was observed between the products of the viral and cellular src genes. It has previously been demonstrated that pp60v-src is associated with a detergent-insoluble matrix containing the cellular cytoskeleton (J. G. Burr, G. Dreyfuss, S. Penman, and J. M. Buchanan, Proc. Natl. Acad. Sci. USA 77:3484-3488, 1980). We observed a similar association of the transforming proteins of Fujinami sarcoma virus (P130gag-fps) and Yamaguchi 73 avian sarcoma virus (P90gag-yes), both of which are tyrosine-specific protein kinases. However, we found that the endogenous c-src product, pp60c-src, was not tightly bound to the detergent-insoluble matrix. This does not appear to have been due to differences in the cytoskeleton between transformed and nontransformed cells since pp60c-src was also solubilized by nonionic detergent in cells transformed by Rous sarcoma virus. This difference in the affinities of the v-src and c-src products for cytoskeletal proteins may contribute to the inability of pp60c-src to transform cells.

MeSH Terms
Avian Sarcoma Viruses Cell Compartmentation Cytoskeleton/metabolism Detergents Myristic Acid Myristic Acids/metabolism Oncogene Protein pp60(v-src) Protein-Tyrosine Kinases/metabolism Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins pp60(c-src) Retroviridae Proteins/metabolism Solubility Time Factors
Chemicals
Detergents Myristic Acids Proto-Oncogene Proteins Retroviridae Proteins Myristic Acid Protein-Tyrosine Kinases Oncogene Protein pp60(v-src) Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Loeb D M
Woolford J
Beemon K
References (58)
58 references, click to expand
  1. Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction.
    J Supramol Struct. 1976;5(2):119-30 PMID: 1034175
  2. Cell transformation by pp60c-src mutated in the carboxy-terminal regulatory domain.
    Cell. 1987 Apr 10;49(1):83-91 PMID: 3103927
  3. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  4. Identification of a transformation-specific antigen induced by an avian sarcoma virus.
    Nature. 1977 Sep 22;269(5626):346-8 PMID: 198667
  5. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  6. Characterization of a normal avian cell protein related to the avian sarcoma virus transforming gene product.
    Cell. 1978 Dec;15(4):1363-9 PMID: 83198
  7. Comparison of the expression of the src gene of Rous sarcoma virus in vitro and in vivo.
    J Virol. 1978 Dec;28(3):957-71 PMID: 215787
  8. Effects of the src gene product on microfilament and microtubule organization in avian and mammalian cells infected with the same temperature-sensitive mutant of Rous sarcoma virus.
    Virology. 1979 Jan 15;92(1):201-10 PMID: 217162
  9. Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product.
    Cell. 1979 Jan;16(1):11-24 PMID: 217542
  10. Uninfected vertebrate cells contain a protein that is closely related to the product of the avian sarcoma virus transforming gene (src).
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1804-8 PMID: 221907
  11. The outer boundary of the cytoskeleton: a lamina derived from plasma membrane proteins.
    Cell. 1979 Aug;17(4):859-65 PMID: 573666
  12. Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1979 Sep;18(1):125-34 PMID: 228858
  13. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  14. Association of the src gene product of Rous sarcoma virus with cytoskeletal structures of chicken embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3484-8 PMID: 6251461
  15. Adhesion plaques of Rous sarcoma virus-transformed cells contain the src gene product.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3514-8 PMID: 6251464
  16. The protein encoded by the transforming gene of avian sarcoma virus (pp60src) and a homologous protein in normal cells (pp60proto-src) are associated with the plasma membrane.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3783-7 PMID: 6253989
  17. Structural and functional domains of the Rous sarcoma virus transforming protein (pp60src).
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1624-8 PMID: 6262821
  18. Transforming proteins of some feline and avian sarcoma viruses are related structurally and functionally.
    Cell. 1981 Apr;24(1):145-53 PMID: 6263483
  19. Organization of pp60src and selected cytoskeletal proteins within adhesion plaques and junctions of Rous sarcoma virus-transformed rat cells.
    J Cell Biol. 1981 Jun;89(3):525-35 PMID: 6265469
  20. Localization of the Abelson murine leukemia virus protein in a detergent-insoluble subcellular matrix: architecture of the protein.
    J Virol. 1981 Nov;40(2):472-81 PMID: 6172595
  21. Changes in amino-terminal sequences of pp60src lead to decreased membrane association and decreased in vivo tumorigenicity.
    Cell. 1982 Apr;28(4):889-96 PMID: 6284379
  22. Two structurally and functionally different forms of the transforming protein of PRC II avian sarcoma virus.
    Mol Cell Biol. 1982 Aug;2(8):890-6 PMID: 6290871
  23. Immunofluorescent localization of the transforming protein of Rous sarcoma virus with antibodies against a synthetic src peptide.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5322-6 PMID: 6291037
  24. Site-directed mutagenesis of the src gene of Rous sarcoma virus: construction and characterization of a deletion mutant temperature sensitive for transformation.
    J Virol. 1982 Nov;44(2):683-91 PMID: 6292526
  25. Polyoma virus middle T antigen: relationship to cell membranes and apparent lack of ATP-binding activity.
    Mol Cell Biol. 1982 Oct;2(10):1187-98 PMID: 6184609
  26. Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus.
    Cell. 1983 Mar;32(3):881-90 PMID: 6299580
  27. Cytoplasmic localization of the transforming protein of Fujinami sarcoma virus: salt-sensitive association with subcellular components.
    J Virol. 1983 Feb;45(2):782-91 PMID: 6300435
  28. Antibodies to a defined region of pp60src neutralize the tyrosine-specific kinase activity.
    J Biol Chem. 1983 Sep 25;258(18):11219-28 PMID: 6411728
  29. Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.
    J Virol. 1983 Nov;48(2):352-60 PMID: 6312092
  30. Subcellular localization of pp60src in RSV-transformed cells.
    Curr Top Microbiol Immunol. 1983;107:51-124 PMID: 6321110
  31. Expression of v-src and chicken c-src in rat cells demonstrates qualitative differences between pp60v-src and pp60c-src.
    Cell. 1984 May;37(1):131-9 PMID: 6426802
  32. Transforming proteins of fujinami and PRCII avian sarcoma viruses have different subcellular locations.
    Virology. 1984 May;135(1):168-80 PMID: 6328747
  33. Rous sarcoma virus variants that carry the cellular src gene instead of the viral src gene cannot transform chicken embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4424-8 PMID: 6087323
  34. Hormonal regulation of the Rous sarcoma virus src gene via a heterologous promoter defines a threshold dose for cellular transformation.
    Cell. 1984 Oct;38(3):757-65 PMID: 6091899
  35. Enhancement of cellular src gene product associated tyrosyl kinase activity following polyoma virus infection and transformation.
    Cell. 1984 Oct;38(3):767-77 PMID: 6091900
  36. Cellular localization of the transforming protein of wild-type and temperature-sensitive Fujinami sarcoma virus.
    J Virol. 1984 Nov;52(2):557-65 PMID: 6092677
  37. Cellular localization of c-fps gene product NCP98.
    J Virol. 1984 Dec;52(3):913-8 PMID: 6092720
  38. Overexpression of the c-src protein does not induce transformation of NIH 3T3 cells.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7071-5 PMID: 6594680
  39. Highly specific antibody to Rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules.
    J Cell Biol. 1985 Feb;100(2):409-17 PMID: 2981886
  40. Fine structural mapping of a critical NH2-terminal region of p60src.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1623-7 PMID: 2984663
  41. Low level of cellular protein phosphorylation by nontransforming overproduced p60c-src.
    Mol Cell Biol. 1985 May;5(5):1058-66 PMID: 2582236
  42. Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells.
    Mol Cell Biol. 1985 May;5(5):1073-83 PMID: 2582237
  43. Mutation of NH2-terminal glycine of p60src prevents both myristoylation and morphological transformation.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4625-8 PMID: 2991884
  44. Protein-tyrosine kinases.
    Annu Rev Biochem. 1985;54:897-930 PMID: 2992362
  45. Activation of the pp60c-src kinase by middle T antigen binding or by dephosphorylation.
    EMBO J. 1985 Jun;4(6):1471-7 PMID: 2411538
  46. Tyr527 is phosphorylated in pp60c-src: implications for regulation.
    Science. 1986 Mar 21;231(4744):1431-4 PMID: 2420005
  47. Association of p60src with Triton X-100-resistant cellular structure correlates with morphological transformation.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2312-6 PMID: 3031677
  48. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  49. Localization of the feline sarcoma virus fgr gene product (P70gag-actin-fgr): association with the plasma membrane and detergent-insoluble matrix.
    J Virol. 1986 Jul;59(1):66-72 PMID: 3012121
  50. Activation of the transforming potential of p60c-src by a single amino acid change.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4228-32 PMID: 2424022
  51. Restriction of the in vitro and in vivo tyrosine protein kinase activities of pp60c-src relative to pp60v-src.
    Mol Cell Biol. 1985 Oct;5(10):2753-63 PMID: 2426575
  52. Altered sites of tyrosine phosphorylation in pp60c-src associated with polyomavirus middle tumor antigen.
    Mol Cell Biol. 1986 May;6(5):1562-70 PMID: 2431281
  53. Avian sarcoma virus gag-fps and gag-yes transforming proteins are not myristylated or palmitylated.
    Virology. 1986 Dec;155(2):716-20 PMID: 3491459
  54. Amino acid substitutions sufficient to convert the nontransforming p60c-src protein to a transforming protein.
    Mol Cell Biol. 1986 Dec;6(12):4155-60 PMID: 2432397
  55. Dephosphorylation or antibody binding to the carboxy terminus stimulates pp60c-src.
    Mol Cell Biol. 1986 Dec;6(12):4467-77 PMID: 2432403
  56. A tail of two src's: mutatis mutandis.
    Cell. 1987 Apr 10;49(1):1-4 PMID: 3030562
  57. Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation.
    Cell. 1987 Apr 10;49(1):65-73 PMID: 3103925
  58. A cytoskeletal structure with associated polyribosomes obtained from HeLa cells.
    Cell. 1977 Jan;10(1):67-78 PMID: 837445
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.8.2420-2427.1987
Published
1987-08-00
Pages
2420-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255659
Subset
IM
Grants
NCI NIH HHS · CA33199 · United States
NCRR NIH HHS · S07 RR07041 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.8.2420-2427.1987 PMC Full Text View journal details
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