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PMID: 2431281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Altered sites of tyrosine phosphorylation in pp60c-src associated with polyomavirus middle tumor antigen.

Molecular and cellular biology ·Vol. 6 ·No. 5 ·1986-05-00 ·Pages 1562-70

Cartwright CA, Kaplan PL, Cooper JA, Hunter T, Eckhart W

Abstract

We characterized the tyrosine phosphorylation sites of free pp60c-src and of pp60c-src associated with the polyomavirus middle tumor antigen (mT) in transformed avian and rodent cells. The sites of tyrosine phosphorylation in the two populations of pp60c-src were different, both in vitro and in vivo. Free pp60c-src was phosphorylated in vitro at a single site, tyrosine 416. pp60c-src associated with mT was phosphorylated in vitro on tyrosine 416 and on one or more additional tyrosine residues located in the amino-terminal region of the molecule. Free pp60c-src in polyomavirus mT-transformed cells was phosphorylated in vivo on tyrosine 527. In contrast, pp60c-src associated with mT was phosphorylated in vivo on tyrosine 416 and not detectably on tyrosine 527. Thus, the in vivo phosphorylation sites of pp60c-src associated with mT in transformed cells are identical to those of pp60v-src, the Rous sarcoma virus transforming protein. The results suggest that altered phosphorylation of pp60c-src associated with mT may play a role in the enhancement of the pp60c-src protein kinase activity and in cell transformation by polyomavirus.

MeSH Terms
Amino Acids/analysis Animals Antibodies, Monoclonal Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/metabolism Cell Transformation, Neoplastic Chick Embryo Leukemia Virus, Murine/genetics Mice Oncogene Proteins, Viral/metabolism Peptide Mapping Phosphorylation Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins pp60(c-src) Trypsin Tyrosine
Chemicals
Amino Acids Antibodies, Monoclonal Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Oncogene Proteins, Viral Proto-Oncogene Proteins Tyrosine Proto-Oncogene Proteins pp60(c-src) Trypsin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cartwright C A
Kaplan P L
Cooper J A
Hunter T
Eckhart W
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52 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.5.1562-1570.1986
Published
1986-05-00
Pages
1562-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367682
Subset
IM
Grants
NCI NIH HHS · CA-01040 · United States
NCI NIH HHS · CA-13884 · United States
NCI NIH HHS · CA-14195 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.5.1562-1570.1986 PMC Full Text View journal details
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