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PMID: 2995972 Published · ppublish English Journal Article

Isolation and chromosomal localization of the human fgr protooncogene, a distinct member of the tyrosine kinase gene family.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 19 ·1985-10-00 ·Pages 6595-9

Tronick SR, Popescu NC, Cheah MS, Swan DC, Amsbaugh SC, Lengel CR, DiPaolo JA, Robbins KC

Abstract

The cell-derived domain of Gardner-Rasheed feline sarcoma virus (GR-FeSV) consists of a gamma-actin- and a tyrosine-specific protein kinase-encoding sequence designated v-fgr. By utilizing a v-fgr probe, it was possible to detect related sequences present at low copy number in DNAs of a variety of mammalian species and to isolate a human fgr homologue. Comparative studies revealed that this human DNA clone represented all but 200 base pairs of v-fgr. Analysis of human genomic DNA demonstrated that the fgr protooncogene was distinct from the cellular homologues of other retrovirus onc genes. In addition, the fgr protooncogene was localized to the distal portion of the short arm of human chromosome 1 at p36.1-36.2 by in situ hybridization. Taken together, our findings establish that the fgr protooncogene is a unique member of the tyrosine kinase gene family.

MeSH Terms
Actins/genetics Animals Cats/genetics Chromosome Mapping Chromosomes, Human, 1-3 DNA, Recombinant Genes, Viral Humans Macaca mulatta/genetics Neoplasms/genetics Oncogenes Phylogeny Proto-Oncogene Proteins/genetics Proto-Oncogenes RNA, Messenger/biosynthesis Raccoons/genetics Retroviridae Proteins/genetics Sarcoma Viruses, Feline/enzymology,genetics Sequence Homology, Nucleic Acid Species Specificity
Chemicals
Actins DNA, Recombinant Proto-Oncogene Proteins RNA, Messenger Retroviridae Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Tronick S R
Popescu N C
Cheah M S
Swan D C
Amsbaugh S C
Lengel C R
DiPaolo J A
Robbins K C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.19.6595
Published
1985-10-00
Pages
6595-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391256
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.19.6595 PMC Full Text View journal details
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