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PMID: 88866 Published · ppublish English Journal Article

Glial fibrillary acidic protein and intermediate filaments in human glioma cells.

Acta neuropathologica ·Vol. 47 ·No. 1 ·1979-06-15 ·Pages 71-4

Paetau A, Virtanen I, Stenman S, Kurki P, Linder E, Vaheri A, Westermark B, Dahl D, Haltia M

Abstract

Cultured human glioma cells were studied by double indirect immunofluorescence technique using antisera against intermediate filaments and glial fibrillary acidic protein. With both antisera cytoplasmic fibrillar fluorescence was seen. Perinuclear bundles of intermediate-sized filaments, induced by vinblastine treatment, were strongly stained with both antisera. The degree of codistribution of the two types of antigenic determinants varied considerably from cell to cell. The results suggest that two types of filament-related antigenic determinants can be present in the same cell, and also that glial fibrillary acidic protein-related filaments may possess functional similarities to the intermediate filaments found in other cells. Glial fibrillary acidic protein remains as a useful and specific antigenic marker for the study of glial cells in vitro.

MeSH Terms
Astrocytoma Brain Neoplasms/analysis Cell Line Epitopes Fluorescent Antibody Technique Glioma/analysis Humans Nerve Tissue Proteins/analysis Neuroglia Vinblastine
Chemicals
Epitopes Nerve Tissue Proteins Vinblastine
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Paetau A
Virtanen I
Stenman S
Kurki P
Linder E
Vaheri A
Westermark B
Dahl D
Haltia M
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33 references, click to expand
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Article Info
Journal
Acta neuropathologica
Abbr.
Acta Neuropathol
ISSN
0001-6322
DOI
10.1007/BF00698276
Published
1979-06-15
Pages
71-4
Language
English
Region
Germany
NLM ID
0412041
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00698276 View journal details
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