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PMID: 8887546 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Endoderm induction by the organizer-secreted factors chordin and noggin in Xenopus animal caps.

The EMBO journal ·Vol. 15 ·No. 17 ·1996-09-02 ·Pages 4547-55

Sasai Y, Lu B, Piccolo S, De Robertis EM

Abstract

Spemann's organizer has potent neural inducing and mesoderm dorsalizing activities in the Xenopus gastrula. A third activity, the organizer's ability to induce a secondary gut, has been difficult to analyze experimentally due to the lack of early gene markers. Here we introduce endodermin, a pan-endodermal gene marker, and use it to demonstrate that chordin (Chd), a protein secreted by the organizer region, is able to induce endodermal differentiation in Xenopus. The ability of chd, as well as that of noggin, to induce endoderm in animal cap explants is repressed by the ventralizing factor BMP-4. When FGF signaling is blocked by a dominant-negative FGF receptor in chd-injected animal caps, neural induction is inhibited and most of the explant is induced to become endoderm. The results suggest that proteins secreted by the organizer, acting together with known peptide growth factors, regulate differentiation of the endodermal germ layer.

MeSH Terms
Amino Acid Sequence Animals Carrier Proteins Cell Differentiation Embryonic Induction Endoderm/cytology Fibroblast Growth Factors/metabolism Gene Expression Regulation, Developmental/physiology Genetic Markers Glycoproteins/genetics Intercellular Signaling Peptides and Proteins Molecular Sequence Data Proteins/genetics Signal Transduction Xenopus/embryology
Chemicals
Carrier Proteins Genetic Markers Glycoproteins Intercellular Signaling Peptides and Proteins Proteins noggin protein Fibroblast Growth Factors chordin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sasai Y
Department of Biological Chemistry, University of California, Los Angeles 90095-1737, USA.
Lu B
Piccolo S
De Robertis E M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-09-02
Pages
4547-55
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452184
Subset
IM
Grants
NICHD NIH HHS · HD21502-11 · United States
Databases
GENBANK
L63543
External Links
PubMed source PMC Full Text View journal details
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