Home LiteratureArticle Details
PMID: 89019 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction of human antigen-specific and non-specific helper factors in vitro.

Clinical and experimental immunology ·Vol. 36 ·No. 1 ·1979-04-00 ·Pages 71-7

Kantor F, Feldmann M

Abstract

Human peripheral blood leucocytes, cultured for 4--6 days with the appropriate concentration of protein or synthetic polypeptide antigen, yield helper cells. These cells, after a further 24 hr incubation with the appropriate antigen release helper factors, which are of two types, antigen specific and non-specific; a similar situation to that described in the mouse. These factors are assayed by their effects on the plaque-forming cell response of mouse spleen cells cultures in vitro. The potential applications of this new assay for human T cell function are discussed.

MeSH Terms
Adult Albumins/immunology Animals Antigens/immunology Cells, Cultured Epitopes Haptens Hemocyanins/immunology Hemolytic Plaque Technique Humans Lymphocyte Cooperation Mice Mice, Inbred C57BL Mice, Inbred CBA Middle Aged Peptides/immunology T-Lymphocytes/immunology
Chemicals
Albumins Antigens Epitopes Haptens Peptides Hemocyanins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kantor F
Feldmann M
References (28)
28 references, click to expand
  1. Induction of an antibody response in cultures of human peripheral blood lymphocytes.
    J Exp Med. 1976 Sep 1;144(3):573-85 PMID: 1085334
  2. Antigen-specific T-cell factor in cell cooperation. Mapping within the I region of the H-2 complex and ability to cooperate across allogeneic barriers.
    J Exp Med. 1975 Sep 1;142(3):694-700 PMID: 1100764
  3. In vivo suppression of delayed hypersensitivity: prolongation of desensitization in guinea pigs.
    J Exp Med. 1975 Sep 1;142(3):588-99 PMID: 1165470
  4. The association between the HLA system and disease.
    Prog Med Genet. 1977;2:39-100 PMID: 142998
  5. Current concepts of the antibody response: heterogeneity of lymphoid cells, interactions, and factors.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:113-8 PMID: 302185
  6. Suppressive and enhancing T-cell factors as I-region gene products: properties and the subregion assignment.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:119-27 PMID: 302186
  7. Regulation of cellular and humoral immune responses by T-cell subclasses.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:23-32 PMID: 302190
  8. Primary in vitro sensitisation of human T cells.
    Nature. 1977 Sep 8;269(5624):151-3 PMID: 302915
  9. Restricted helper function of F1 hybrid T cells positively selected to heterologous erythrocytes in irradiated parental strain mice. I. Failure to collaborate with B cells of the opposite parental strain not associated with active suppression.
    J Exp Med. 1978 Apr 1;147(4):1142-58 PMID: 306407
  10. Cell interactions between histoincompatible T and B lymphocytes. II. Failure of physiologic cooperative interactions between T and B lymphocytes from allogeneic donor strains in humoral response to hapten-protein conjugates.
    J Exp Med. 1973 Jun 1;137(6):1405-18 PMID: 4122709
  11. Primary immune response in cultures of spleen cells.
    Lancet. 1967 Dec 16;2(7529):1279-81 PMID: 4168614
  12. Induction of antibody formation and tolerance in vitro to a purified protein antigen.
    Lancet. 1967 Dec 16;2(7529):1281-5 PMID: 4168615
  13. Regulation of homocytotropic antibody formation in the rat. X. IgT-like molecule for the induction of homocytotropic antibody response.
    J Immunol. 1974 Dec;113(6):1757-69 PMID: 4430832
  14. Induction of specific helper cells in vitro.
    Nat New Biol. 1973 Oct 31;245(148):285-6 PMID: 4543295
  15. Biological expressions of lymphocyte activation. II. Generation of a population of thymus-derived suppressor lymphocytes.
    J Exp Med. 1973 Mar 1;137(3):649-59 PMID: 4570017
  16. Inhibitory and stimulatory effects of concanavalin A on the response of mouse spleen cell suspensions to antigen. I. Characterization of the inhibitory cell activity.
    J Exp Med. 1972 Dec 1;136(6):1445-60 PMID: 4641854
  17. HL-A and disease associations--a survey.
    Transplant Rev. 1975;22:3-43 PMID: 46644
  18. Two genes in the major histocompatibility complex control immune response.
    Nature. 1975 Jul 10;256(5513):103-6 PMID: 50559
  19. Cell interactions in the immune response in vitro. 3. Specific collaboration across a cell impermeable membrane.
    J Exp Med. 1972 Jul 1;136(1):49-67 PMID: 5064225
  20. The xenogeneic effect. I. Antigen and mitogen-stimulated human lymphocytes produce a non-antigen-specific factor which reconstitutes the antibody response of T cell-deficient mouse spleen cells.
    J Immunol. 1975 Nov;115(5):1295-1300 PMID: 51888
  21. Further improvements in the plaque technique for detecting single antibody-forming cells.
    Immunology. 1968 Apr;14(4):599-600 PMID: 5653817
  22. Immunogenicity of trinitrophenyl-hemocyanin: production of primary and secondary anti-hapten precipitins.
    J Immunol. 1966 Sep;97(3):421-30 PMID: 5925708
  23. Chemical coupling of proteins to agarose.
    Nature. 1967 Sep 30;215(5109):1491-2 PMID: 6059570
  24. Antigen-specific T cell factors: a fine analysis of specificity.
    J Immunol. 1977 Feb;118(2):584-8 PMID: 65430
  25. T-T interactions in the induction of suppressor and helper T cells: analysis of membrane phenotype of precursor and amplifier cells.
    J Exp Med. 1977 Apr 1;145(4):793-801 PMID: 67169
  26. Education of human lymphocytes against mouse cells: specific recognition of H-2 antigens.
    Eur J Immunol. 1977 Feb;7(2):81-5 PMID: 68884
  27. In vitro studies on H-2-linked unresponsiveness to synthetic polypeptides. III. Production of an antigen-specific T helper cell factor to (T,G)-A--L.
    Eur J Immunol. 1977 Jul;7(7):417-21 PMID: 70359
  28. Detection of acceptor sites on human lymphocytes for antigen-specific T cell factors.
    Nature. 1977 Nov 10;270(5633):151-4 PMID: 73139
Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1979-04-00
Pages
71-7
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1537693
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2