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PMID: 8909255 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A human colon carcinoma cell line exhibits adhesive interactions with P-selectin under fluid flow via a PSGL-1-independent mechanism.

The American journal of pathology ·Vol. 149 ·No. 5 ·1996-11-00 ·Pages 1661-73

Goetz DJ, Ding H, Atkinson WJ, Vachino G, Camphausen RT, Cumming DA, Luscinskas FW

Abstract

It has been postulated that endothelial cell adhesion molecules involved in leukocyte recruitment play a role in metastasis. Using an in vitro flow model, we studied the adhesion of the human colon carcinoma cell line KM12-L4 to P-selectin, an inducible endothelial-expressed adhesion molecule involved in leukocyte recruitment. Recombinant forms of P-selectin and Chinese hamster ovary cells stably expressing P-selectin supported attachment and rolling of KM12-L4 cells at 1 to 2 dynes/cm2. The adhesive interactions to P-selectin were abolished by pretreatment of the KM12-L4 cells with neuraminidase but were unaltered by pretreatment of the KM12-L4 cells with O-sialoglycoprotein endopeptidase, an enzyme that cleaves mucin type glycoproteins such as P-selectin glycoprotein ligand-1 (PSGL-1). PSGL-1 is the only counter-receptor for P-selectin known to mediate myeloid cell adhesion to P-selectin under flow. Flow cytometric and Northern blot analyses revealed that KM12-L4 cells did not express PSGL-1 and monoclonal antibody PL1, a function-blocking monoclonal antibody to PSGL-1, had no inhibitory effect on KM12-L4 adhesion to P-selectin under flow. Compared with HL-60 cells, which express PSGL-1, the KM12-L4 cells exhibited a slightly lower rate of attachment to P-selectin and rolled at a significantly higher velocity. In summary, KM12-L4 human colon carcinoma cells interact with P-selectin, under flow, through a PSGL-1-independent adhesion pathway.

MeSH Terms
Animals Antibodies, Monoclonal/immunology CHO Cells Carcinoma/metabolism Cell Adhesion/drug effects,physiology Colonic Neoplasms/metabolism Cricetinae E-Selectin/metabolism Flow Cytometry Humans Immunoglobulin G/metabolism Membrane Glycoproteins/immunology,pharmacology Metalloendopeptidases N-Acetylneuraminic Acid/metabolism P-Selectin/metabolism Recombinant Fusion Proteins Rheology Tumor Cells, Cultured
Chemicals
Antibodies, Monoclonal E-Selectin Immunoglobulin G Membrane Glycoproteins P-Selectin P-selectin ligand protein Recombinant Fusion Proteins Metalloendopeptidases O-sialoglycoprotein endopeptidase N-Acetylneuraminic Acid
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Goetz D J
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Ding H
Atkinson W J
Vachino G
Camphausen R T
Cumming D A
Luscinskas F W
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1996-11-00
Pages
1661-73
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1865285
Subset
IM
Grants
NHLBI NIH HHS · HL36028 · United States
NHLBI NIH HHS · HL47646 · United States
NHLBI NIH HHS · T32HL07627 · United States
External Links
PubMed source PMC Full Text View journal details
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