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PMID: 893660 Published · ppublish English Journal Article

Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains.

Journal of clinical microbiology ·Vol. 6 ·No. 2 ·1977-08-00 ·Pages 96-100

Ljungh A, Popoff M, Wadstrom T

Abstract

Eleven isolates of Aeromonas species from human stool cultures were found to produce enterotoxin activity as determined by assay of culture filtrates in rabbit intestinal loops and rabbit skin and on adrenal Y1 cells. Hemolysin(s) and a cytotoxic protein were found to interfere in all three assay systems but could be inactivated upon heating at 56 degrees C or by specific antihemolysin. Biotyping of each isolate was performed with a conventional test system and with API 50E and APIZYM kit systems (Analytab, Inc.). No single test of the more than 70 biochemical reactions investigated was found to correlate with enterotoxigenicity in the strains of Aeromonas hydrophila examined. All strains were found to belong to ideal phenotypes of A. hydrophila, but each strain possessed its own biochemical profile.

MeSH Terms
Aeromonas/enzymology,metabolism Arginine/metabolism Carbohydrate Metabolism Child Diarrhea/microbiology Enterotoxins/biosynthesis Hemolysis Histidine/metabolism Humans Hydrolases/metabolism Species Specificity
Chemicals
Enterotoxins Histidine Arginine Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ljungh A
Popoff M
Wadstrom T
References (28)
28 references, click to expand
  1. Enterotoxigenic Escherichia coli isolated from food.
    J Infect Dis. 1977 Feb;135(2):313-7 PMID: 320279
  2. Isolation of skin permeability factors from culture filtrates of Salmonella typhimurium.
    Infect Immun. 1976 Sep;14(3):671-9 PMID: 786890
  3. Enterotoxin-producing bacteria and parasites in stools of Ethiopian children with diarrhoeal disease.
    Arch Dis Child. 1976 Nov;51(11):865-70 PMID: 1008593
  4. Plesiomonas shigelloides. Survey and possible association with diarrhoea in Zaïre.
    Ann Soc Belg Med Trop. 1974;54(6):503-13 PMID: 4469970
  5. Induction of steroidogenesis in tissue culture by cholera enterotoxin.
    Nat New Biol. 1973 Jun 20;243(129):246-7 PMID: 4351987
  6. Purification and some properties of a heat-labile enterotoxin from escherichia coli.
    Zentralbl Bakteriol Orig A. 1974;229(2):190-204 PMID: 4155864
  7. Characterization of three Aeromonas and nine pseudomonas species by extracellular enzymes and haemolysins.
    Med Microbiol Immunol. 1975;161(2):79-87 PMID: 1170482
  8. A taxonomic study of the Aeromonas hydrophila-Aeromonas punctata group.
    J Gen Microbiol. 1976 May;94(1):11-22 PMID: 932684
  9. Fixation and inactivation of cholera toxin by GM1 ganglioside.
    Scand J Infect Dis. 1973;5(1):77-8 PMID: 4199382
  10. Letter: Cholera syndrome in West Bengal.
    Lancet. 1976 Feb 7;1(7954):317 PMID: 55642
  11. Three characteristics associated with enterotoxigenic Escherichia coli isolated from man.
    Infect Immun. 1973 Sep;8(3):322-8 PMID: 4581006
  12. Cross-reactivity between heat labile enterotoxins of Vibrio cholerae and Escherichia coli in neutralization tests in rabbit ileum and skin.
    Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):757-62 PMID: 4592974
  13. Tissue-culture assay of antibodies to heat-liable Escherichia coli enterotoxins.
    N Engl J Med. 1974 Jul 18;291(3):117-21 PMID: 4600168
  14. Stimulation of jejunal secretion by a crude Escherichia coli enterotixin.
    Gastroenterology. 1972 Sep;63(6):439-48 PMID: 4562844
  15. The pathogenesis of Shigella diarrhea. V. Relationship of shiga enterotoxin, neurotoxin, and cytotoxin.
    J Infect Dis. 1975 May;131 Suppl:S33-9 PMID: 236346
  16. The characterization and significance of Plesiomonas shigelloides and Aeromonas hydrophila isolated from an epidemic of diarrhoea.
    Indian J Med Res. 1974 Jul;62(7):1051-60 PMID: 4435916
  17. The detection of Aeromonas hydrophila in stool specimens.
    Health Lab Sci. 1970 Jul;7(3):124-7 PMID: 4915588
  18. Role of toxigenic and invasive bacteria in acute diarrhea of childhood.
    N Engl J Med. 1975 Sep 18;293(12):567-72 PMID: 1097914
  19. Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides.
    J Med Microbiol. 1975 Feb;8(1):195-8 PMID: 1092866
  20. Reproducibility of the analytab (API 20E) system.
    J Clin Microbiol. 1975 Oct;2(4):322-6 PMID: 1102558
  21. Human diarrheal disease caused by enterotoxigenic Escherichia coli.
    Annu Rev Microbiol. 1975;29:333-53 PMID: 1101807
  22. Enterotoxin, haemolysin and cytotoxic protein in Aeromonas hydrophila from human infections.
    Acta Pathol Microbiol Scand B. 1976 Apr;84(2):112-4 PMID: 1274594
  23. False-positive rabbit ileal loop reactions attributed to Vibrio parahaemolyticus broth filtrates.
    J Infect Dis. 1976 Apr;133(4):436-40 PMID: 1262710
  24. [Nutrition and taxonomy of "Enterobacteriaceae" and related bacteria. I. Technical procedure for auxanograms (author's transl)].
    Ann Microbiol (Paris). 1975 Apr;126(3):267-74 PMID: 1190636
  25. Cytotoxicity of Vibrio parahaemolyticus in HeLa cell culture.
    J Infect Dis. 1975 Nov;132(5):555-60 PMID: 1185016
  26. Separation of two hemolysins from Aeromonas hydrophila by isoelectric focusing.
    Infect Immun. 1971 Oct;4(4):503-5 PMID: 5154892
  27. An enterotoxin of Pseudomonas aeruginosa.
    J Infect Dis. 1971 Jan;123(1):97-8 PMID: 5543221
  28. The genus aeromonas in human bacteriology; report of 30 cases and review of the literature.
    N Engl J Med. 1968 Feb 1;278(5):245-9 PMID: 5635458
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.6.2.96-100.1977
Published
1977-08-00
Pages
96-100
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC274713
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.6.2.96-100.1977 PMC Full Text View journal details
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