Home LiteratureArticle Details
PMID: 8943337 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Recombination induced by triple-helix-targeted DNA damage in mammalian cells.

Molecular and cellular biology ·Vol. 16 ·No. 12 ·1996-12-00 ·Pages 6820-8

Faruqi AF, Seidman MM, Segal DJ, Carroll D, Glazer PM

Abstract

Gene therapy has been hindered by the low frequency of homologous recombination in mammalian cells. To stimulate recombination, we investigated the use of triple-helix-forming oligonucleotides (TFOs) to target DNA damage to a selected site within cells. By treating cells with TFOs linked to psoralen, recombination was induced within a simian virus 40 vector carrying two mutant copies of the supF tRNA reporter gene. Gene conversion events, as well as mutations at the target site, were also observed. The variety of products suggests that multiple cellular pathways can act on the targeted damage, and data showing that the triple helix can influence these pathways are presented. The ability to specifically induce recombination or gene conversion within mammalian cells by using TFOs may provide a new research tool and may eventually lead to novel applications in gene therapy.

MeSH Terms
Animals Cell Line DNA Damage Genetic Vectors Mammals Mutation Nucleic Acid Conformation Oligonucleotides/genetics Recombination, Genetic
Chemicals
Oligonucleotides
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Faruqi A F
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA.
Seidman M M
Segal D J
Carroll D
Glazer P M
References (51)
51 references, click to expand
  1. Repair of DNA containing interstrand crosslinks in Escherichia coli: sequential excision and recombination.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1064-8 PMID: 4577788
  2. Use of psoralen-modified oligonucleotides to trap three-stranded RecA-DNA complexes and repair of these cross-linked complexes by ABC excinuclease.
    J Biol Chem. 1988 Oct 15;263(29):15110-7 PMID: 2844801
  3. Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: the RAD3 system and the RAD51 system.
    Mol Gen Genet. 1981;182(2):196-205 PMID: 7026973
  4. Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3153-7 PMID: 6328502
  5. Psoralens as photoactive probes of nucleic acid structure and function: organic chemistry, photochemistry, and biochemistry.
    Annu Rev Biochem. 1985;54:1151-93 PMID: 2411210
  6. High frequency targeting of genes to specific sites in the mammalian genome.
    Cell. 1986 Feb 14;44(3):419-28 PMID: 3002636
  7. Action mechanism of ABC excision nuclease on a DNA substrate containing a psoralen crosslink at a defined position.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8077-81 PMID: 3534882
  8. Analysis of sequential steps of nucleotide excision repair in Escherichia coli using synthetic substrates containing single psoralen adducts.
    J Biol Chem. 1988 Nov 15;263(32):16553-60 PMID: 3053693
  9. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.
    Nature. 1988 Nov 24;336(6197):348-52 PMID: 3194019
  10. Specificity in formation of triple-stranded nucleic acid helical complexes: studies with agarose-linked polyribonucleotide affinity columns.
    Biochemistry. 1988 Dec 27;27(26):9108-12 PMID: 3242616
  11. In vitro repair of psoralen-DNA cross-links by RecA, UvrABC, and the 5'-exonuclease of DNA polymerase I.
    J Biol Chem. 1989 Apr 25;264(12):6755-65 PMID: 2708342
  12. Incision by UvrABC excinuclease is a step in the path to mutagenesis by psoralen crosslinks in Escherichia coli.
    Proc Natl Acad Sci U S A. 1989 Jun;86(11):3982-6 PMID: 2657732
  13. Inhibition of DNA binding proteins by oligonucleotide-directed triple helix formation.
    Science. 1989 Aug 18;245(4919):725-30 PMID: 2549631
  14. DNA interstrand cross-links promote chromosomal integration of a selected gene in human cells.
    Mol Cell Biol. 1989 Jul;9(7):2897-905 PMID: 2779552
  15. Intermolecular recombination between DNAs introduced into mouse L cells is mediated by a nonconservative pathway that leads to crossover products.
    Mol Cell Biol. 1990 Jan;10(1):103-12 PMID: 2294396
  16. Homologous recombination in mammalian cells.
    Annu Rev Genet. 1989;23:199-225 PMID: 2694931
  17. Frequency of intrachromosomal homologous recombination induced by UV radiation in normally repairing and excision repair-deficient human cells.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1566-70 PMID: 2154752
  18. Sequence-specific artificial photo-induced endonucleases based on triple helix-forming oligonucleotides.
    Nature. 1990 Mar 22;344(6264):358-60 PMID: 2156170
  19. Characterization of nonconservative homologous junctions in mammalian cells.
    Mol Cell Biol. 1990 Dec;10(12):6613-8 PMID: 2174111
  20. Second structural motif for recognition of DNA by oligonucleotide-directed triple-helix formation.
    Science. 1991 Mar 15;251(4999):1360-3 PMID: 2003222
  21. RecA-dependent incision of psoralen-crosslinked DNA by (A)BC excinuclease.
    Nucleic Acids Res. 1991 Feb 11;19(3):657-63 PMID: 2011535
  22. Involvement of single-stranded tails in homologous recombination of DNA injected into Xenopus laevis oocyte nuclei.
    Mol Cell Biol. 1991 Jun;11(6):3268-77 PMID: 2038330
  23. Removal of psoralen monoadducts and crosslinks by human cell free extracts.
    Nucleic Acids Res. 1991 Sep 11;19(17):4623-9 PMID: 1891353
  24. Evidence that a triplex-forming oligodeoxyribonucleotide binds to the c-myc promoter in HeLa cells, thereby reducing c-myc mRNA levels.
    Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8227-31 PMID: 1896473
  25. Site-specific cleavage of human chromosome 4 mediated by triple-helix formation.
    Science. 1991 Dec 13;254(5038):1639-42 PMID: 1836279
  26. Specific inhibition of transcription by triple helix-forming oligonucleotides.
    Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):504-8 PMID: 1731320
  27. Human nucleotide excision nuclease removes thymine dimers from DNA by incising the 22nd phosphodiester bond 5' and the 6th phosphodiester bond 3' to the photodimer.
    Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3664-8 PMID: 1314396
  28. The role and fate of DNA ends for homologous recombination in embryonic stem cells.
    Mol Cell Biol. 1992 Jun;12(6):2464-74 PMID: 1588950
  29. Psoralen damage-induced plasmid recombination in Saccharomyces cerevisiae: dependence on RAD1 and RAD52.
    Mutat Res. 1992 Jun;274(1):1-9 PMID: 1375327
  30. A signature element distinguishes sibling and independent mutations in a shuttle vector plasmid.
    Gene. 1992 Aug 1;117(1):1-5 PMID: 1644298
  31. Inhibition of gene expression by triple helix-directed DNA cross-linking at specific sites.
    Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3501-5 PMID: 8475098
  32. In vivo transcription of a progesterone-responsive gene is specifically inhibited by a triplex-forming oligonucleotide.
    Nucleic Acids Res. 1993 Jun 25;21(12):2789-96 PMID: 8332487
  33. Targeted mutagenesis of DNA using triple helix-forming oligonucleotides linked to psoralen.
    Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7879-83 PMID: 8356097
  34. Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide.
    J Virol. 1993 Dec;67(12):7324-31 PMID: 8230456
  35. Mismatch repair of heteroduplex DNA intermediates of extrachromosomal recombination in mammalian cells.
    Mol Cell Biol. 1994 Jan;14(1):400-6 PMID: 8264607
  36. Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells.
    Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6064-8 PMID: 8016116
  37. Site-specific targeting of psoralen photoadducts with a triple helix-forming oligonucleotide: characterization of psoralen monoadduct and crosslink formation.
    Nucleic Acids Res. 1994 Jul 25;22(14):2845-52 PMID: 8052539
  38. Analysis of biological selections for high-efficiency gene targeting.
    Mol Cell Biol. 1995 Jan;15(1):45-51 PMID: 7799954
  39. Pulsed-field gel electrophoresis analysis of the repair of psoralen plus UVA induced DNA photoadducts in Saccharomyces cerevisiae.
    Mutat Res. 1995 Jan;336(1):49-60 PMID: 7528895
  40. Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes.
    Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):806-10 PMID: 7846056
  41. Targeted mutagenesis in mammalian cells mediated by intracellular triple helix formation.
    Mol Cell Biol. 1995 Mar;15(3):1759-68 PMID: 7862165
  42. Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiae.
    Mol Cell Biol. 1995 Apr;15(4):1968-73 PMID: 7891691
  43. Altered repair of targeted psoralen photoadducts in the context of an oligonucleotide-mediated triple helix.
    J Biol Chem. 1995 Sep 22;270(38):22595-601 PMID: 7673252
  44. Triple helix directed psoralen adducts induce a low frequency of recombination in an SV40 shuttle vector.
    Biochim Biophys Acta. 1995 Sep 19;1263(3):235-40 PMID: 7548210
  45. Mutagenesis by third-strand-directed psoralen adducts in repair-deficient human cells: high frequency and altered spectrum in a xeroderma pigmentosum variant.
    Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2941-6 PMID: 8610147
  46. Mutagenesis in mammalian cells induced by triple helix formation and transcription-coupled repair.
    Science. 1996 Feb 9;271(5250):802-5 PMID: 8628995
  47. Sequence-specific cleavage of double helical DNA by triple helix formation.
    Science. 1987 Oct 30;238(4827):645-50 PMID: 3118463
  48. Carcinogens can induce homologous recombination between duplicated chromosomal sequences in mouse L cells.
    Mol Cell Biol. 1988 Jan;8(1):196-202 PMID: 3122022
  49. Sequence-specific binding and photocrosslinking of alpha and beta oligodeoxynucleotides to the major groove of DNA via triple-helix formation.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1349-53 PMID: 3422738
  50. Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro.
    Science. 1988 Jul 22;241(4864):456-9 PMID: 3293213
  51. Initiation of genetic exchanges in lambda phage--prophage crosses.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):291-5 PMID: 264683
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.12.6820
Published
1996-12-00
Pages
6820-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231685
Subset
IM
Grants
NCI NIH HHS · CA64186 · United States
NIEHS NIH HHS · ES05775 · United States
External Links
PubMed source DOI: 10.1128/MCB.16.12.6820 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2