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PMID: 8943341 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry.

Molecular and cellular biology ·Vol. 16 ·No. 12 ·1996-12-00 ·Pages 6859-69

Pestova TV, Hellen CU, Shatsky IN

Abstract

Translation of picornavirus RNA is initiated after ribosomal binding to an internal ribosomal entry site (IRES) within the 5' untranslated region. We have reconstituted IRES-mediated initiation on encephalomyocarditis virus RNA from purified components and used primer extension analysis to confirm the fidelity of 48S preinitiation complex formation. Eukaryotic initiation factor 2 (eIF2), eIF3, and eIF4F were required for initiation; eIF4B and to a lesser extent the pyrimidine tract-binding protein stimulated this process. We show that eIF4F binds to the IRES in a novel cap-independent manner and suggest that cap- and IRES-dependent initiation mechanisms utilize different modes of interaction with this factor to promote ribosomal attachment to mRNA.

MeSH Terms
Base Sequence Eukaryotic Initiation Factor-2/genetics,metabolism Eukaryotic Initiation Factor-4F Molecular Sequence Data Peptide Initiation Factors/genetics,metabolism Picornaviridae/genetics RNA, Messenger/genetics RNA, Viral/genetics Ribosomes/genetics
Chemicals
Eukaryotic Initiation Factor-2 Eukaryotic Initiation Factor-4F Peptide Initiation Factors RNA, Messenger RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pestova T V
Department of Microbiology and Immunology, Morse Institute for Molecular Genetics, State University of New York Health Science Center at Brooklyn, 11203-2098, USA.
Hellen C U
Shatsky I N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.12.6859
Published
1996-12-00
Pages
6859-69
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231689
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.12.6859 PMC Full Text View journal details
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