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PMID: 8945596 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae.

Infection and immunity ·Vol. 64 ·No. 12 ·1996-12-00 ·Pages 5406-9

Sperandio V, Bailey C, Girón JA, DiRita VJ, Silveira WD, Vettore AL, Kaper JB

Abstract

The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin-positive and -negative V. cholerae O1 and O139 strains.

MeSH Terms
Adhesins, Bacterial/genetics Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics Base Sequence Cloning, Molecular Molecular Sequence Data Vibrio cholerae/genetics
Chemicals
Adhesins, Bacterial Bacterial Outer Membrane Proteins OmpU protein, Vibrio cholerae
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sperandio V
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore 21201, USA.
Bailey C
Girón J A
DiRita V J
Silveira W D
Vettore A L
Kaper J B
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.12.5406-5409.1996
Published
1996-12-00
Pages
5406-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC174538
Subset
IM
Grants
NIAID NIH HHS · AI19716 · United States
NIAID NIH HHS · AI31645 · United States
Databases
GENBANK
U73751
External Links
PubMed source DOI: 10.1128/iai.64.12.5406-5409.1996 PMC Full Text View journal details
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