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PMID: 8962144 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 25 ·1996-12-10 ·Pages 14851-6

Chen T, Gotschlich EC

Abstract

Neisseria gonorrhoeae (GC) or Escherichia coli expressing phase-variable opacity (Opa) protein (Opa+ GC or Opa+ E. coli) adhere to human neutrophils and stimulate phagocytosis, whereas their counterparts not expressing Opa protein (Opa- GC or Opa- E. coli) do not. Opa+ GC or E. coli do not adhere to human lymphocytes and promyelocytic cell lines such as HL-60 cells. The adherence of Opa+ GC to the neutrophils can be enhanced dramatically if the neutrophils are preactivated. These data suggest that the components binding the Opa+ bacteria might exist in the granules. CGM1a antigen, a transmembrane protein of the carcinoembryonic antigen family, is exclusively expressed in the granulocytic lineage. The predicted molecular weight of CGM1a is approximately 30 kDa. We observed specific binding of OpaI+ E. coli to a 30-kDa band of polymorphonuclear leukocytes lysates. To prove the hypothesis that the 30-kDa CGM1a antigen from neutrophils was the receptor of Opa+ bacteria, we showed that a HeLa cell line expressing human CGM1a antigen (HeLa-CGM1a) bound Opa+ E. coli and subsequently engulfed the bacteria. Monoclonal antibodies (COL-1) against CGM1 blocked the interaction between Opa+ E. coli and HeLa-CGM1a. These results demonstrate that HeLa cells when expressing the CGM1a antigens bind and internalize OpaI+ bacteria.

MeSH Terms
Antigens, Bacterial/metabolism Bacterial Adhesion Bacterial Outer Membrane Proteins/metabolism Carcinoembryonic Antigen/metabolism Cell Adhesion Molecules/metabolism Escherichia coli HeLa Cells Humans Neutrophils/metabolism
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins CEACAM3 protein, human Carcinoembryonic Antigen Cell Adhesion Molecules opacity proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chen T
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY 10021, USA.
Gotschlich E C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.25.14851
Published
1996-12-10
Pages
14851-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC26225
Subset
IM
Grants
NIAID NIH HHS · AI 10615 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.25.14851 PMC Full Text View journal details
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