Home LiteratureArticle Details
PMID: 896462 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Complex forms and replicative intermediates of mitochondrial DNA in tissues from adult and senescent mice.

Nucleic acids research ·Vol. 4 ·No. 5 ·1977-00-00 ·Pages 1301-14

Pikó L, Matsumoto L

Abstract

The occurrence and types of complex forms and replicative intermediates of mitochondrial DNA (mtDNA) were investigated in tissues from C57BL/6J mice aged 10-11 months or 29-30 months. Total mtDNA from brain, heart, kidney and liver was isolated in ethidium bromide-CsCl gradients and examined by electron microscopy after aqueous or formamide spreading. Contour length measurements indicated no difference in the monomer size of mtDNA according to either tissue or donor age. The frequencies of catenated mtDNA, ranging from 4 to 8%, varied significantly according to tissue but changed relatively little as a result of donor age. The main age-related effect observed in this study was a significant increase in the frequency of circular dimers, from about 0.05% in adult tissues to 0.3% in kidney, 0.5% in liver, 0.6% in heart and 1.9% in brain of senescent mice. The frequency of D-loop DNA varied from 30 to 60% and that of larger replicative intermediates from 1 to 10%, suggesting differences in the rate of mtDNA replication according to tissue. The frequencies and types of the various replicative intermediates were unaffected by donor age.

MeSH Terms
Adult Aged Aging Animals Brain/growth & development,metabolism DNA Replication DNA, Circular DNA, Mitochondrial/biosynthesis Heart/growth & development Humans Kidney/growth & development,metabolism Liver/growth & development Male Mice Mice, Inbred C57BL Microscopy, Electron Mitochondria, Liver/metabolism Mitochondria, Muscle/metabolism Molecular Weight Nucleic Acid Conformation Organ Specificity
Chemicals
DNA, Circular DNA, Mitochondrial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pikó L
Matsumoto L
References (38)
38 references, click to expand
  1. Mechanism of mitochondrial DNA replication in mouse L-cells: asynchronous replication of strands, segregation of circular daughter molecules, aspects of topology and turnover of an initiation sequence.
    J Mol Biol. 1974 Jul 15;86(4):801-24 PMID: 4473554
  2. Cleavage of replicating forms of mitochondrial DNA by EcoRI endonuclease.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4447-51 PMID: 4612520
  3. Replication of mitochondrial DNA. Circular replicative intermediates in mouse L cells.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):737-41 PMID: 4501588
  4. A novel closed-circular mitochondrial DNA with properties of a replicating intermediate.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2252-7 PMID: 5289384
  5. Abnormal DNA patterns in animal mitochondria: ethidium bromide-induced breakdown of closed circular DNA and conditions leading to oligomer accumulation.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1926-33 PMID: 4321352
  6. Reversible generation of circular dimer and higher multiple forms of mitochondrial DNA.
    Nature. 1969 Sep 13;223(5211):1124-9 PMID: 5810682
  7. Multiple length DNA molecules of bacteriophage phi-X174.
    Nat New Biol. 1972 May 31;237(74):141-4 PMID: 4504192
  8. Ageing and the regulation of cell activities during exposure to cold.
    J Gen Physiol. 1969 Dec;54(6):690-712 PMID: 4391050
  9. Replication of mitochondrial DNA: replicative forms of molecules from rat tissues and evidence for discontinuous replication.
    Cold Spring Harb Symp Quant Biol. 1974;38:267-80 PMID: 4364784
  10. Degenerative changes in the mitochondria of flight muscle from aging blowflies.
    J Cell Biol. 1972 Feb;52(2):465-77 PMID: 4333452
  11. Regulation of mitochondrial respiration in senescence.
    J Cell Physiol. 1972 Aug;80(1):141-8 PMID: 4341986
  12. Hematologic and serum electrolyte values of the C57BL-6J male mouse in maturity and senescence.
    Lab Anim Sci. 1973 Jun;23(3):339-49 PMID: 4351468
  13. Mechanism of mitochondrial DNA replication in mouse L-cells: topology of circular daughter molecules and dynamics of catenated oligomer formation.
    J Mol Biol. 1976 Jan 5;100(1):85-92 PMID: 1249842
  14. Replication of circular DNA in eukaryotic cells.
    Annu Rev Biochem. 1974;43(0):695-719 PMID: 4605048
  15. The regulation of physiological changes during mammalian aging.
    Q Rev Biol. 1976 Mar;51(1):49-83 PMID: 6991
  16. Mitochondrial nucleic acids.
    Annu Rev Biochem. 1972;41:333-76 PMID: 4563438
  17. Deletion mutants of bacteriophage phiX174.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1905-10 PMID: 4558663
  18. Complex mitochondrial DNA in human tumors.
    Cancer Res. 1973 May;33(5):1065-70 PMID: 4512952
  19. Electron microscope studies of heteroduplex DNA from a deletion mutant of bacteriophage phiX-174.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1948-52 PMID: 4505672
  20. Circular oligomers in mitochondrial DNA of human and beef nonmalignant thyroid glands.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):847-50 PMID: 4502936
  21. Age-related changes in the flight muscle mitochondria from the blowfly Sarcophaga bullata.
    J Gerontol. 1976 May;31(3):257-63 PMID: 178708
  22. The effect of age on mitochondrial ultrastructure and enzyme cytochemistry.
    Biochem Soc Trans. 1975;3(1):126-8 PMID: 165103
  23. Complex mitochondrial DNA.
    Int Rev Exp Pathol. 1975;14:1-67 PMID: 170231
  24. The role of recombination in the formation of circular oligomers of the lambda plasmid.
    J Mol Biol. 1974 Sep 5;88(1):65-87 PMID: 4444039
  25. Mitochondrial DNA replication in sea urchin oocytes.
    J Cell Biol. 1974 Oct;63(1):146-59 PMID: 4418353
  26. Enlarged seminal vesicles of senescent C57BL-6J mice.
    J Gerontol. 1974 Mar;29(2):134-8 PMID: 4811948
  27. 5-bromodeoxyuridine labeling of monomeric and catenated circular mitochondrial DNA in HeLa cells.
    J Mol Biol. 1973 Feb 25;74(2):81-94 PMID: 4689958
  28. Catecholamine metabolism in the brains of ageing male mice.
    Brain Res. 1973 Mar 30;52:261-76 PMID: 4700706
  29. Specific cleavage analysis of mammalian mitochondrial DNA.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4496-500 PMID: 1060130
  30. Complex mitochondrial DNA in animal thyroids. A comparative study.
    Biochim Biophys Acta. 1976 May 19;432(3):257-66 PMID: 1268254
  31. Number of mitochondria and some properties of mitochondrial DNA in the mouse egg.
    Dev Biol. 1976 Mar;49(1):1-10 PMID: 943339
  32. A fine structural study of degenerative changes in the dorsal column nuclei of aging mice. Lack of protection by vitamin E.
    J Gerontol. 1975 Jul;30(4):395-411 PMID: 1141671
  33. Cytoplasmic DNA in the unfertilized sea urchin egg: physical properties of circular mitochondrial DNA and the occurrence of catenated forms.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):838-45 PMID: 5238664
  34. Complex mitochondrial DNA in leukemic and normal human myeloid cells.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1077-84 PMID: 5256408
  35. Apparent turnover of mitochondrial deoxyribonucleic acid and mitochondrial phospholipids in the tissues of the rat.
    J Biol Chem. 1969 Mar 25;244(6):1552-62 PMID: 5773057
  36. Occurrence of complex mitochondrial DNA in normal tissues.
    Nature. 1968 Dec 7;220(5171):976-9 PMID: 5701854
  37. Circular dimer and catenate forms of mitochondrial DNA in human leukaemic leucocytes.
    Nature. 1967 Nov 18;216(5116):652-7 PMID: 6082459
  38. Catenated circular DNA molecules in HeLa cell mitochondria.
    Nature. 1967 Nov 18;216(5116):647-52 PMID: 6082458
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.5.1301
Published
1977-00-00
Pages
1301-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343757
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.5.1301 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2