Home LiteratureArticle Details
PMID: 8996252 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Follicular dendritic cells specifically express the long CR2/CD21 isoform.

The Journal of experimental medicine ·Vol. 185 ·No. 1 ·1997-01-06 ·Pages 165-70

Liu YJ, Xu J, de Bouteiller O, Parham CL, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore KW

Abstract

This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, CD/biosynthesis,genetics B-Lymphocytes/immunology Base Sequence Child Cloning, Molecular DNA Primers DNA, Complementary Dendritic Cells/immunology Exons Humans L Cells Mice Mice, Inbred BALB C Molecular Sequence Data Palatine Tonsil/immunology Polymerase Chain Reaction Receptors, Complement 3d/biosynthesis,genetics Recombinant Proteins/biosynthesis Transfection
Chemicals
Antibodies, Monoclonal Antigens, CD DNA Primers DNA, Complementary Receptors, Complement 3d Recombinant Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Liu Y J
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Xu J
de Bouteiller O
Parham C L
Grouard G
Djossou O
de Saint-Vis B
Lebecque S
Banchereau J
Moore K W
References (30)
30 references, click to expand
  1. Follicular dendritic cells as accessory cells.
    Immunol Rev. 1990 Oct;117:185-211 PMID: 2258191
  2. Follicular dendritic cells help resting B cells to become effective antigen-presenting cells: induction of B7/BB1 and upregulation of major histocompatibility complex class II molecules.
    J Exp Med. 1993 Dec 1;178(6):2055-66 PMID: 7504055
  3. Intraclonal generation of antibody mutants in germinal centres.
    Nature. 1991 Dec 5;354(6352):389-92 PMID: 1956400
  4. Germinal centres in T-cell-dependent antibody responses.
    Immunol Today. 1992 Jan;13(1):17-21 PMID: 1739427
  5. A receptor for interleukin 10 is related to interferon receptors.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11267-71 PMID: 8248239
  6. Isolation of a cDNA encoding thymic shared antigen-1. A new member of the Ly6 family with a possible role in T cell development.
    J Immunol. 1993 Dec 15;151(12):6913-23 PMID: 8258699
  7. Generation of memory B cells and plasma cells in vitro.
    Science. 1995 May 5;268(5211):720-2 PMID: 7537388
  8. The CD19/CR2/TAPA-1 complex of B lymphocytes: linking natural to acquired immunity.
    Annu Rev Immunol. 1995;13:127-49 PMID: 7542009
  9. Human follicular dendritic cells enhance cytokine-dependent growth and differentiation of CD40-activated B cells.
    J Immunol. 1995 Oct 1;155(7):3345-52 PMID: 7561028
  10. C3d of complement as a molecular adjuvant: bridging innate and acquired immunity.
    Science. 1996 Jan 19;271(5247):348-50 PMID: 8553069
  11. Within germinal centers, isotype switching of immunoglobulin genes occurs after the onset of somatic mutation.
    Immunity. 1996 Mar;4(3):241-50 PMID: 8624814
  12. Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen.
    Immunity. 1996 Mar;4(3):251-62 PMID: 8624815
  13. Dendritic cells: features and functions.
    Immunol Rev. 1980;53:127-47 PMID: 6162776
  14. Production of a monoclonal antibody reactive with human dendritic reticulum cells and its use in the immunohistological analysis of lymphoid tissue.
    J Clin Pathol. 1983 Feb;36(2):167-75 PMID: 6338047
  15. Peroxidase-positive mononuclear leukocytes as possible precursors of human dendritic reticulum cells.
    J Immunol. 1983 Dec;131(6):2719-25 PMID: 6358354
  16. Molecular cloning of the cDNA encoding the Epstein-Barr virus/C3d receptor (complement receptor type 2) of human B lymphocytes.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9194-8 PMID: 2827171
  17. Structure of the human B lymphocyte receptor for C3d and the Epstein-Barr virus and relatedness to other members of the family of C3/C4 binding proteins.
    J Exp Med. 1988 Mar 1;167(3):1047-66 PMID: 2832506
  18. Role of IgD and T delta cells in the regulation of the humoral immune response.
    Immunol Rev. 1988 Oct;105:45-67 PMID: 3058578
  19. Genomic organization and polymorphisms of the human C3d/Epstein-Barr virus receptor.
    J Biol Chem. 1989 Feb 5;264(4):2118-25 PMID: 2563370
  20. Structure and function of the complement receptors, CR1 (CD35) and CR2 (CD21).
    Adv Immunol. 1989;46:183-219 PMID: 2551147
  21. Antibody-forming cell induction during an early phase of germinal centre development and its delay with ageing.
    Immunology. 1989 Nov;68(3):312-8 PMID: 2592007
  22. Mechanism of antigen-driven selection in germinal centres.
    Nature. 1989 Dec 21-28;342(6252):929-31 PMID: 2594086
  23. In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody.
    J Exp Med. 1990 Aug 1;172(2):665-8 PMID: 1695671
  24. Genes for Plasmodium falciparum surface antigens cloned by expression in COS cells.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6363-7 PMID: 1696728
  25. Maturation of the immune response in germinal centers.
    Cell. 1991 Dec 20;67(6):1121-9 PMID: 1760840
  26. Antigens in immunity. XV. Ultrastructural features of antigen capture in primary and secondary lymphoid follicles.
    J Exp Med. 1968 Feb 1;127(2):277-90 PMID: 4169585
  27. CD21 is a ligand for CD23 and regulates IgE production.
    Nature. 1992 Aug 6;358(6386):505-7 PMID: 1386409
  28. Follicular dendritic cells and B cell costimulation.
    J Immunol. 1993 Jan 1;150(1):31-8 PMID: 8417129
  29. Direct evidence that human follicular dendritic cells (FDC) rescue germinal centre B cells from death by apoptosis.
    Clin Exp Immunol. 1993 Feb;91(2):330-6 PMID: 8428399
  30. Suppression of the immune response by a soluble complement receptor of B lymphocytes.
    Science. 1991 Oct 4;254(5028):102-5 PMID: 1718035
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.185.1.165
Published
1997-01-06
Pages
165-70
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2196095
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.185.1.165 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2