Expression of interferon-alpha receptor mRNA in the liver in chronic liver diseases associated with hepatitis C virus: relation to effectiveness of interferon therapy.

Journal of gastroenterology ·Vol. 31 ·No. 6 ·1996-12-00 ·Pages 806-11

Fukuda R, Ishimura N, Ishihara S, Tokuda A, Satoh S, Sakai S, Akagi S, Watanabe M, Fukumoto S

Abstract

To investigate whether interferon-alpha receptor (IFN-alpha Rc) expression was related to the effectiveness of interferon therapy in hepatitis C virus (HCV)-associated chronic liver disease (CLD). IFN-alpha Rc mRNA was investigated by reverse transcription polymerase chain reaction (RT-PCR) in liver biopsies and peripheral blood mononuclear cells (PBMCs) from 40 patients with HCV-associated CLD who subsequently received IFN-alpha therapy. IFN-alpha Rc mRNA in the liver was detected in 18 of 20 (90%) responders to IFN and in 5 of 20 (25%) non-responders (P < 0.01). In PBMCs, IFN-alpha Rc mRNA was detected in all patients regardless of response to IFN. Increased histological hepatitis activity and liver fibrosis were significantly related to the absence of IFN-alpha Rc mRNA. The HCV-RNA genotype showed no significant relationship to IFN-alpha Rc mRNA expression. Our results suggest that IFN-alpha Rc mRNA expression in the liver, but not in PBMCs, is closely associated with the effectiveness of IFN-alpha therapy in HCV-associated CLD.

MeSH Terms

Antiviral Agents/administration & dosage,therapeutic use Biopsy Chronic Disease Disease Progression Female Follow-Up Studies Gene Expression Genotype Hepacivirus/genetics Hepatitis C/metabolism,pathology,therapy Humans Interferon alpha-2 Interferon-alpha/administration & dosage,therapeutic use Liver/metabolism Male Middle Aged Oligonucleotide Probes/chemistry Polymerase Chain Reaction Predictive Value of Tests RNA, Messenger/biosynthesis RNA, Viral/analysis Receptor, Interferon alpha-beta Receptors, Interferon/biosynthesis,genetics Recombinant Proteins Treatment Outcome

Chemicals

Antiviral Agents Interferon alpha-2 Interferon-alpha Oligonucleotide Probes RNA, Messenger RNA, Viral Receptors, Interferon Recombinant Proteins Receptor, Interferon alpha-beta

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Fukuda R

Second Department of Internal Medicine, Shimane Medical University, Japan.

Ishimura N

Ishihara S

Tokuda A

Satoh S

Sakai S

Akagi S

Watanabe M

Fukumoto S

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Article Info

Journal

Journal of gastroenterology

Abbr.

J Gastroenterol

ISSN

0944-1174

DOI

10.1007/BF02358606

Published

1996-12-00

Pages

806-11

Language

English

Region

Japan

NLM ID

9430794

Subset

External Links

PubMed source DOI: 10.1007/BF02358606 View journal details

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Expression of interferon-alpha receptor mRNA in the liver in chronic liver diseases associated with hepatitis C virus: relation to effectiveness of interferon therapy.

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