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PMID: 90333 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Staphylococcal nuclease reviewed: a prototypic study in contemporary enzymology. IV. The nuclease as a model for protein folding.

Molecular and cellular biochemistry ·Vol. 23 ·No. 3 ·1979-02-09 ·Pages 131-41

Tucker PW, Hazen EE, Cotton FA

Abstract

This is the last in a series of four articles in which the chemical, enzymological and crystallographic work on Ribonucleate (deoxyribonucleate)-3'-nucleotidohydrolase, EC 3.1.4.4 (staphylococcal nuclease, micrococcal nuclease) will be reviewed and correlated. This article discusses the use of the nuclease as a model system for the study of the mechanisms and energetics of the folding-unfolding reaction in proteins and for the study of the interrelationships between amino acid sequence and three-dimensional structure.

MeSH Terms
Epitopes Hydrogen-Ion Concentration Kinetics Ligands Micrococcal Nuclease/immunology,metabolism Models, Chemical Peptide Fragments Protein Conformation Protein Denaturation Thermodynamics
Chemicals
Epitopes Ligands Peptide Fragments Micrococcal Nuclease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tucker P W
Hazen E E
Cotton F A
References (40)
40 references, click to expand
  1. Dynamics of the helix--coil transition in poly-L-ornithine.
    J Am Chem Soc. 1969 Mar 26;91(7):1812-6 PMID: 5779828
  2. Immunological distinction between the possible origins of enzymatic activity in a polypeptide fragment of staphylococcal nuclease.
    Nature. 1974 Sep 20;251(5472):242-4 PMID: 4138651
  3. Protein denaturation. C. Theoretical models for the mechanism of denaturation.
    Adv Protein Chem. 1970;24:1-95 PMID: 4912353
  4. Semisynthetic analogues of an enzymically active complex formed between two overlapping fragments of staphylococcal nuclease.
    J Biol Chem. 1971 Dec 10;246(23):7392-7 PMID: 5127932
  5. Tentative sequential model for the unfolding and refolding of staphylococcal nuclease at high pH.
    Cold Spring Harb Symp Quant Biol. 1972;36:257-61 PMID: 4343718
  6. Binding of univalent antibody fragments to a distinct antigenic determinant of staphylococcal nuclease.
    Biochemistry. 1974 Apr 9;13(8):1567-71 PMID: 4133940
  7. Synthetic studies of structure-function relationships in staphylococcal nuclease. Synthetic analogues of fragment P2.
    J Biol Chem. 1969 Dec 10;244(23):6316-22 PMID: 5354957
  8. A high resolution structure of an inhibitor complex of the extracellular nuclease of Staphylococcus aureus. I. Experimental procedures and chain tracing.
    J Biol Chem. 1971 Apr 10;246(7):2302-16 PMID: 5555571
  9. Immunochemistry of staphylococcal nuclease. I. Physical, enzymatic, and immunological studies of chemically modified derivatives.
    Biochemistry. 1970 Jan 20;9(2):304-12 PMID: 4983881
  10. Nuclear magnetic resonance studies of the structure and binding sites of enzymes. XIV. Inhibitor binding to staphylococcal nuclease.
    J Mol Biol. 1970 Jun 14;50(2):223-33 PMID: 5476914
  11. Purification and properties of semisynthetic staphylococcal nuclease-T'.
    J Biol Chem. 1971 May 10;246(9):2948-52 PMID: 4324344
  12. Study of equilibration of the system involving two alternative, enzymically active complementing structures simultaneously formed from two overlapping fragments of staphylococcal nuclease.
    J Biol Chem. 1977 Jan 10;252(1):125-40 PMID: 833112
  13. Nuclear magnetic resonance studies of the structure and binding sites of enzymes. XI. Characterization of selectively deuterated analogs of staphylococcal nuclease.
    Proc Natl Acad Sci U S A. 1970 Feb;65(2):395-401 PMID: 4984238
  14. Chemical studies of structural features in staphylococcal nuclease-T'.
    J Biol Chem. 1972 Apr 10;247(7):1999-2007 PMID: 4335858
  15. Nuclease-T: an active derivative of staphylococcal nuclease composed of two noncovalently bonded peptide fragments.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):1235-42 PMID: 5233844
  16. The dynamic equilibrium of folding and unfolding of nuclease-T.
    J Biol Chem. 1973 Jul 25;248(14):5164-74 PMID: 4352194
  17. An immunologic approach to the conformational equilibria of polypeptides.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3790-4 PMID: 4118873
  18. A solid phase synthetic study of structure-function relationships in the amino-terminal region of staphylococcal nuclease.
    J Biol Chem. 1970 Sep 25;245(18):4718-23 PMID: 5456145
  19. Staphylococcal nuclease reviewed: a prototypic study in contemporary enzymology. I. Isolation; physical and enzymatic properties.
    Mol Cell Biochem. 1978 Dec 22;22(2-3):67-77 PMID: 370553
  20. Ligand-induced resistance of staphylococcal nuclease and nuclease-T to proteolysis by subtilisin, alpha-chymotrypsin, and thermolysin.
    J Biol Chem. 1969 Sep 10;244(17 ):4600-6 PMID: 4309147
  21. An experimental approach to the study of the folding of staphylococcal nuclease.
    J Biol Chem. 1969 Jul 25;244(14 ):3864-75 PMID: 4308739
  22. Experimental and theoretical aspects of protein folding.
    Adv Protein Chem. 1975;29:205-300 PMID: 237413
  23. Antibodies to a distinct antigenic determinant of staphylococcal nuclease.
    J Immunol. 1972 Dec;109(6):1300-10 PMID: 4117550
  24. Principles that govern the folding of protein chains.
    Science. 1973 Jul 20;181(4096):223-30 PMID: 4124164
  25. NMR relaxation studies of the unfolding and refolding of staphylococcal nuclease at low pH.
    Ann N Y Acad Sci. 1973 Dec 31;222:230-9 PMID: 4361856
  26. Staphylococcal nuclease reviewed: a prototypic study in contemporary enzymology. II. Solution studies of the nucleotide binding site and the effects of nucleotide binding.
    Mol Cell Biochem. 1979 Jan 15;23(1):3-16 PMID: 423893
  27. Proton magnetic resonance studies at 220 MHz of the histidine residues of staphylococcal nuclease.
    Nature. 1970 Nov 14;228(5272):642-4 PMID: 5474936
  28. Steps in the formation of active derivatives of staphylococcal nuclease during trypsin digestion.
    J Biol Chem. 1968 Sep 25;243(18):4778-86 PMID: 5687720
  29. A kinetic study of the complementation of fragments of staphylococcal nuclease.
    J Biol Chem. 1974 Apr 10;249(7):2285-93 PMID: 4362071
  30. The specific binding of three fragments of staphylococcal nuclease.
    J Biol Chem. 1971 Dec 25;246(24):7421-8 PMID: 4332553
  31. The occurrence of autoacceleration during hydrolysis of nucleic acids by micrococcal nuclease.
    J Biol Chem. 1968 Sep 25;243(18):4917-21 PMID: 4301228
  32. The structural basis of the catalytic function of staphylococcal nuclease.
    Brookhaven Symp Biol. 1968 Jun;21(1):172-200 PMID: 4305102
  33. Simultaneous formation of two alternative enzymology active structures by complementation of two overlapping fragments of staphylococcal nuclease.
    J Biol Chem. 1971 Apr 10;246(7):2291-301 PMID: 5555570
  34. The mechanism of stabilization of the structure of nuclease-T by binding of ligands.
    J Biol Chem. 1975 Mar 25;250(6):2388-94 PMID: 163827
  35. Immunochemistry of staphylococcal nuclease. II. Inhibition and binding studies with sequence fragments.
    Biochemistry. 1970 Jan 20;9(2):313-21 PMID: 4189660
  36. The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. I. Linear order of the fragments produced by cleavage with cyanogen bromide.
    J Biol Chem. 1966 Oct 10;241(19):4366-85 PMID: 5922961
  37. Kinetics of folding of staphylococcal nuclease.
    Science. 1970 Feb 6;167(3919):886-7 PMID: 5410853
  38. Inactivation of staphylococcal nuclease by the binding of antibodies to a distinct antigenic determinant.
    Biochemistry. 1972 Nov 7;11(23):4268-73 PMID: 4116930
  39. A comparison of the x-ray diffraction patterns of crystals of reconstituted nuclease-T and of native staphylococcal nuclease.
    J Biol Chem. 1972 May 25;247(10 ):3362-4 PMID: 4337514
  40. Some aspects of the structure of Staphylococcal nuclease. II. Studies in solution.
    Cold Spring Harb Symp Quant Biol. 1972;36:249-55 PMID: 4343717
Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00219452
Published
1979-02-09
Pages
131-41
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00219452 View journal details
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