Polynucleotides. XLV Synthesis and properties of poly(2'-azido-2'-deoxyinosinic acid).

Nucleic acids research ·Vol. 4 ·No. 8 ·1977-08-00 ·Pages 2629-39

Fukui T, Kakiuchi N, Ikehara M

Abstract

Poly (2'-azido-2'-deoxyinosinic acid), [poly (Iz)], was synthesized from 2'-azido-2'-deoxyinosine diphosphate by the action of polynucleotide phosphorylase. Poly (Iz) has UV absorption properties similar to poly (I) and hypochromicity of 11% at 0.15M Na+ and neutrality. In solutions of high Na+ ion concentration, poly (Iz) forms a multi-stranded complex and its Tm at 1.0M Na+ ion concentration was 43 degrees. Upon mixing with poly (C), poly (Iz) forms a 1:1 complex having a Tm lower than that of poly (I)-poly (C) complex in the same conditions. The effect of substitution at the 2'-position of the poly (I) strand was discussed in relation to the interferon-inducing activity.

MeSH Terms

Azides Chemical Phenomena Chemistry Circular Dichroism Inosine Nucleotides Kinetics Macromolecular Substances Nucleic Acid Conformation Osmolar Concentration Poly C Polydeoxyribonucleotides/biosynthesis Polyribonucleotide Nucleotidyltransferase/metabolism Spectrophotometry, Ultraviolet Temperature

Chemicals

Azides Inosine Nucleotides Macromolecular Substances Polydeoxyribonucleotides Poly C Polyribonucleotide Nucleotidyltransferase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Fukui T

Kakiuchi N

Ikehara M

References (16)

16 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.8.2629

Published

1977-08-00

Pages

2629-39

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342597

Subset

External Links

PubMed source DOI: 10.1093/nar/4.8.2629 PMC Full Text View journal details

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Polynucleotides. XLV Synthesis and properties of poly(2'-azido-2'-deoxyinosinic acid).

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