Structure of transcriptionally-active chromatin subunits.

Nucleic acids research ·Vol. 4 ·No. 9 ·1977-09-00 ·Pages 3155-73

Gottesfeld JM, Butler PJ

Abstract

Rat liver chromatin is organized into regions of DNA which differ in degree of susceptibility to attack by the endonucleases DNase I and DNase II. The most nuclease-sensitive portion of chromatin DNA is enriched in transcribed sequences. This fraction may be separated from the bulk of chromatin by virtue of its solubility in solutions containing 2 mM MgCl2. Both transcribed and nontranscribed regions of chromatin are organized into repeating units of DNA and histone, which appear as 100 A beads in the electron microscope. The length of DNA in the repeat unit is the same for these two classes of chromatin (198 +/- 6 base pairs in rat liver); however, the subunits of active, Mg++-soluble chromatin differ from the nucleosomes of inactive regions of chromatin in several respects. Active subunits are enriched in nascent RNA and nonhistone protein and exhibit higher sedimentation values than the corresponding subunits of inactive chromatin.

MeSH Terms

Animals Cell Fractionation Cell Nucleus/metabolism Chromatin/metabolism,ultrastructure DNA/metabolism Deoxyribonucleases Kinetics Liver/metabolism Magnesium Micrococcal Nuclease Microscopy, Electron Molecular Weight Rats Transcription, Genetic

Chemicals

Chromatin DNA Deoxyribonucleases Micrococcal Nuclease Magnesium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gottesfeld J M

Butler P J

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30 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.9.3155

Published

1977-09-00

Pages

3155-73

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342641

Subset

External Links

PubMed source DOI: 10.1093/nar/4.9.3155 PMC Full Text View journal details

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