Home LiteratureArticle Details
PMID: 9184215 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

rqh1+, a fission yeast gene related to the Bloom's and Werner's syndrome genes, is required for reversible S phase arrest.

The EMBO journal ·Vol. 16 ·No. 10 ·1997-05-15 ·Pages 2682-92

Stewart E, Chapman CR, Al-Khodairy F, Carr AM, Enoch T

Abstract

In eukaryotic cells, S phase can be reversibly arrested by drugs that inhibit DNA synthesis or DNA damage. Here we show that recovery from such treatments is under genetic control and is defective in fission yeast rqh1 mutants. rqh1+, previously known as hus2+, encodes a putative DNA helicase related to the Escherichia coli RecQ helicase, with particular homology to the gene products of the human BLM and WRN genes and the Saccharomyces cerevisiae SGS1 gene. BLM and WRN are mutated in patients with Bloom's syndrome and Werner's syndrome respectively. Both syndromes are associated with genomic instability and cancer susceptibility. We show that, like BLM and SGS1, rqh1+ is required to prevent recombination and that in fission yeast suppression of inappropriate recombination is essential for reversible S phase arrest.

MeSH Terms
Adenosine Triphosphatases/genetics Amino Acid Sequence Bloom Syndrome/genetics DNA Helicases/genetics DNA Replication Exodeoxyribonucleases Gene Deletion Genes, Fungal Hydroxyurea/pharmacology Mitosis/drug effects,genetics,radiation effects Molecular Sequence Data Multigene Family RecQ Helicases Recombination, Genetic S Phase/genetics Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid Ultraviolet Rays Werner Syndrome/genetics Werner Syndrome Helicase
Chemicals
Schizosaccharomyces pombe Proteins Exodeoxyribonucleases Adenosine Triphosphatases Bloom syndrome protein RECQL protein, human RecQ protein, E coli DNA Helicases RecQ Helicases WRN protein, human Werner Syndrome Helicase Rqh1 protein, S pombe Hydroxyurea
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stewart E
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Chapman C R
Al-Khodairy F
Carr A M
Enoch T
References (44)
44 references, click to expand
  1. Spontaneous and UV-induced recombination in radiation-sensitive mutants of Schizosaccharomyces pombe.
    Mutat Res. 1981 Mar;81(1):37-48 PMID: 7254221
  2. Feedback controls and G2 checkpoints: fission yeast as a model system.
    Bioessays. 1993 Dec;15(12):775-82 PMID: 8141795
  3. DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe.
    Cell. 1987 Sep 11;50(6):917-25 PMID: 3040264
  4. Nucleotide excision repair syndromes: molecular basis and clinical symptoms.
    Philos Trans R Soc Lond B Biol Sci. 1995 Jan 30;347(1319):75-81 PMID: 7746858
  5. Mutation of fission yeast cell cycle control genes abolishes dependence of mitosis on DNA replication.
    Cell. 1990 Feb 23;60(4):665-73 PMID: 2406029
  6. Sgs1: a eukaryotic homolog of E. coli RecQ that interacts with topoisomerase II in vivo and is required for faithful chromosome segregation.
    Cell. 1995 Apr 21;81(2):253-60 PMID: 7736577
  7. Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.
    EMBO J. 1984 Aug;3(8):1737-44 PMID: 6090122
  8. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.
    Nucleic Acids Res. 1989 Jun 26;17(12):4713-30 PMID: 2546125
  9. Creative blocks: cell-cycle checkpoints and feedback controls.
    Nature. 1992 Oct 15;359(6396):599-604 PMID: 1406993
  10. The Schizosaccharomyces pombe rad3 checkpoint gene.
    EMBO J. 1996 Dec 2;15(23):6641-51 PMID: 8978690
  11. A fission yeast RCC1-related protein is required for the mitosis to interphase transition.
    EMBO J. 1994 Feb 1;13(3):606-15 PMID: 8313905
  12. Fission yeast genes involved in coupling mitosis to completion of DNA replication.
    Genes Dev. 1992 Nov;6(11):2035-46 PMID: 1427071
  13. The Bloom's syndrome gene product is homologous to RecQ helicases.
    Cell. 1995 Nov 17;83(4):655-66 PMID: 7585968
  14. DNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe.
    EMBO J. 1992 Apr;11(4):1343-50 PMID: 1563350
  15. Human homologues of yeast helicase.
    Nature. 1996 Oct 24;383(6602):678-9 PMID: 8878475
  16. Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase.
    Mol Cell Biol. 1996 Jul;16(7):3235-44 PMID: 8668138
  17. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe.
    Gene. 1992 May 1;114(1):59-66 PMID: 1587485
  18. 5-Bromodeoxyuridine-dependent increase in sister chromatid exchange formation in Bloom's syndrome is associated with reduction in topoisomerase II activity.
    Exp Cell Res. 1987 Mar;169(1):245-54 PMID: 3028845
  19. Sister chromatid exchange frequencies in Progeria and Werner syndrome patients.
    Am J Hum Genet. 1981 Sep;33(5):762-6 PMID: 7294025
  20. Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast.
    J Cell Sci. 1990 Nov;97 ( Pt 3):509-16 PMID: 2074269
  21. A checkpoint regulates the rate of progression through S phase in S. cerevisiae in response to DNA damage.
    Cell. 1995 Sep 8;82(5):841-7 PMID: 7671311
  22. Positional cloning of the Werner's syndrome gene.
    Science. 1996 Apr 12;272(5259):258-62 PMID: 8602509
  23. Xeroderma pigmentosum, Cockayne syndrome and trichothiodystrophy: do the genes explain the diseases?
    Trends Genet. 1996 May;12(5):187-92 PMID: 8984734
  24. Hydroxyurea treatment does not prevent initiation of DNA synthesis in Ehrlich ascites tumour cells and leads to the accumulation of short DNA fragments containing the replication origins.
    Biochim Biophys Acta. 1984 Feb 24;781(1-2):39-44 PMID: 6696915
  25. Checkpoints: controls that ensure the order of cell cycle events.
    Science. 1989 Nov 3;246(4930):629-34 PMID: 2683079
  26. Cell cycle control of replication initiation in eukaryotes.
    Curr Opin Cell Biol. 1996 Dec;8(6):815-21 PMID: 8939667
  27. Bloom syndrome: a mendelian prototype of somatic mutational disease.
    Medicine (Baltimore). 1993 Nov;72(6):393-406 PMID: 8231788
  28. Identification and characterization of new elements involved in checkpoint and feedback controls in fission yeast.
    Mol Biol Cell. 1994 Feb;5(2):147-60 PMID: 8019001
  29. Spontaneous and induced chromosomal instability in Werner syndrome.
    Hum Genet. 1988 Oct;80(2):135-9 PMID: 2459043
  30. The G1/S cell-cycle checkpoint in eukaryotic cells.
    Biochim Biophys Acta. 1994 Dec 30;1198(2-3):215-30 PMID: 7819276
  31. Cellular responses to DNA damage: cell-cycle checkpoints, apoptosis and the roles of p53 and ATM.
    Trends Biochem Sci. 1995 Oct;20(10):426-30 PMID: 8533157
  32. Mitotic spindle pulls but fails to separate chromosomes in type II DNA topoisomerase mutants: uncoordinated mitosis.
    EMBO J. 1986 May;5(5):1003-10 PMID: 15957215
  33. The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase.
    Mol Cell Biol. 1994 Dec;14(12):8391-8 PMID: 7969174
  34. Chromatid exchanges in ataxia telangiectasia, Bloom syndrome, Werner syndrome, and xeroderma pigmentosum.
    Ann Hum Genet. 1976 Jul;40(1):79-86 PMID: 962324
  35. SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae.
    Genetics. 1996 Nov;144(3):935-45 PMID: 8913739
  36. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
    Methods Enzymol. 1991;194:795-823 PMID: 2005825
  37. DNA topoisomerase II is required at the time of mitosis in yeast.
    Cell. 1985 Jun;41(2):553-63 PMID: 2985283
  38. A large circular minichromosome of Schizosaccharomyces pombe requires a high dose of type II DNA topoisomerase for its stabilization.
    Mol Gen Genet. 1995 Mar 20;246(6):671-9 PMID: 7898434
  39. The ataxia-telangiectasia gene: a link between checkpoint controls, neurodegeneration and cancer.
    Trends Genet. 1995 Oct;11(10):375-7 PMID: 7482757
  40. Cloning and characterisation of the S. pombe rad15 gene, a homologue to the S. cerevisiae RAD3 and human ERCC2 genes.
    Nucleic Acids Res. 1992 Jun 11;20(11):2673-8 PMID: 1319571
  41. Mitotic checkpoint control in fission yeast.
    Cold Spring Harb Symp Quant Biol. 1991;56:409-16 PMID: 1819501
  42. Hypersensitivity of Bloom's syndrome fibroblasts to N-ethyl-N-nitrosourea.
    Mutat Res. 1987 Sep;184(2):147-51 PMID: 3627143
  43. DNA mismatch repair and cancer.
    Gastroenterology. 1995 Nov;109(5):1685-99 PMID: 7557155
  44. Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis.
    EMBO J. 1986 Nov;5(11):2973-9 PMID: 16453724
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.10.2682
Published
1997-05-15
Pages
2682-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1169879
Subset
IM
Grants
NIGMS NIH HHS · GM50015 · United States
Databases
GENBANK
Y09426
External Links
PubMed source DOI: 10.1093/emboj/16.10.2682 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2