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PMID: 9199439 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of bacterial Mn-cofactored superoxide dismutase in oxidative stress responses, nasopharyngeal colonization, and sustained bacteremia caused by Haemophilus influenzae type b.

Infection and immunity ·Vol. 65 ·No. 7 ·1997-07-00 ·Pages 2700-6

D'Mello RA, Langford PR, Kroll JS

Abstract

Haemophilus influenzae type b, a causative agent of bacterial sepsis and meningitis in young children, contains a single superoxide dismutase (SOD), a cytoplasmic MnSOD. To study the role of this enzyme, a chromosomal sodA::lacZ mutant (M-2) was constructed. M-2 had an increased sensitivity towards oxygen and the redox-active agent paraquat. A 3.4-fold increase in sodA-lacZ expression was found in M-2 grown with oxygen supply rates between 3 and 36 mmol of O2/liter/h. In similar experiments with the wild type, assaying SodA activity, a 3.1-fold increase was found. Both the wild type and M-2 grew best at the lowest oxygen supply rate tested, consistent with the notion that H. influenzae prefers a more anaerobic environment. In the infant rat model of infection, the ability of M-2 to colonize the nasopharynx was found to be impaired, but its ability to cause invasive disease was unaffected. This suggests that after invasion, the growth disadvantage imposed by a SodA- phenotype is not limiting.

MeSH Terms
Animals Bacteremia/etiology Haemophilus Infections/microbiology Haemophilus influenzae/growth & development,pathogenicity Mutation Nose/microbiology Oxidative Stress Pharynx/microbiology Rats Superoxide Dismutase/genetics,metabolism Virulence
Chemicals
Superoxide Dismutase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
D'Mello R A
Department of Paediatrics, Imperial College School of Medicine at St. Mary's Hospital, London, United Kingdom.
Langford P R
Kroll J S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.65.7.2700-2706.1997
Published
1997-07-00
Pages
2700-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC175381
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1128/iai.65.7.2700-2706.1997 PMC Full Text View journal details
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